Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts

SS Dhamija, R Gera, DK Singh, D Singh, R Marchant

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Abstract

Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).
LanguageEnglish
Pages307-312
JournalJOURNAL OF BASIC MICROBIOLOGY
Volume37
Issue number5
Publication statusPublished - 1997

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yeasts
Saccharomyces cerevisiae
ethanol production
Kluyveromyces
Kluyveromyces marxianus
pyruvate kinase
alcohol dehydrogenase
molasses
polyacrylamide
isozymes
ethanol
gels

Cite this

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title = "Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts",
abstract = "Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6{\%} (v/v) both at 30 and 45 degrees C as against 3{\%} and 4.2{\%} (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).",
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Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts. / Dhamija, SS; Gera, R; Singh, DK; Singh, D; Marchant, R.

In: JOURNAL OF BASIC MICROBIOLOGY, Vol. 37, No. 5, 1997, p. 307-312.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts

AU - Dhamija, SS

AU - Gera, R

AU - Singh, DK

AU - Singh, D

AU - Marchant, R

PY - 1997

Y1 - 1997

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AB - Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).

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JO - Jounal of Basic Microbiology

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