Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts

SS Dhamija, R Gera, DK Singh, D Singh, R Marchant

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Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).
Original languageEnglish
Pages (from-to)307-312
Issue number5
Publication statusPublished - 1997


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