Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).
|Journal||JOURNAL OF BASIC MICROBIOLOGY|
|Publication status||Published - 1997|
Dhamija, SS., Gera, R., Singh, DK., Singh, D., & Marchant, R. (1997). Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts. JOURNAL OF BASIC MICROBIOLOGY, 37(5), 307-312.