Phloroglucinols from Anti-Microbial Deposit-Resins of Australian Stingless Bees (Tetragonula carbonaria)

C. Flavia Massaro, Thomas J. Smyth, Franklin Smyth, Tim Heard, Sara D. Leonhardt, Mohammad Katouli, Helen M. Wallace, Peter Brooks

    Research output: Contribution to journalArticle

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    Abstract

    Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreportedfor Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crudeextracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.
    LanguageEnglish
    Pages48
    JournalPhytotherapy Research
    Volume29
    Issue number1
    DOIs
    Publication statusPublished - 2014

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    Phloroglucinol
    Propolis
    Bees
    Plant Resins
    Staphylococcus aureus
    Terpenes
    Biological Products
    Gram-Negative Bacteria
    Flavonoids
    Gas Chromatography-Mass Spectrometry
    Pseudomonas aeruginosa
    Agar
    Anti-Bacterial Agents

    Cite this

    Massaro, C. F., Smyth, T. J., Smyth, F., Heard, T., Leonhardt, S. D., Katouli, M., ... Brooks, P. (2014). Phloroglucinols from Anti-Microbial Deposit-Resins of Australian Stingless Bees (Tetragonula carbonaria). Phytotherapy Research, 29(1), 48. https://doi.org/10.1002/ptr.5225
    Massaro, C. Flavia ; Smyth, Thomas J. ; Smyth, Franklin ; Heard, Tim ; Leonhardt, Sara D. ; Katouli, Mohammad ; Wallace, Helen M. ; Brooks, Peter. / Phloroglucinols from Anti-Microbial Deposit-Resins of Australian Stingless Bees (Tetragonula carbonaria). In: Phytotherapy Research. 2014 ; Vol. 29, No. 1. pp. 48.
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    abstract = "Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreportedfor Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crudeextracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.",
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    Massaro, CF, Smyth, TJ, Smyth, F, Heard, T, Leonhardt, SD, Katouli, M, Wallace, HM & Brooks, P 2014, 'Phloroglucinols from Anti-Microbial Deposit-Resins of Australian Stingless Bees (Tetragonula carbonaria)', Phytotherapy Research, vol. 29, no. 1, pp. 48. https://doi.org/10.1002/ptr.5225

    Phloroglucinols from Anti-Microbial Deposit-Resins of Australian Stingless Bees (Tetragonula carbonaria). / Massaro, C. Flavia; Smyth, Thomas J.; Smyth, Franklin; Heard, Tim; Leonhardt, Sara D.; Katouli, Mohammad; Wallace, Helen M.; Brooks, Peter.

    In: Phytotherapy Research, Vol. 29, No. 1, 2014, p. 48.

    Research output: Contribution to journalArticle

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