Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation.

E.M Brown, Gordon J McDougall, Derek Stewart, Gema Pereira-Caro, Rocio González-Barrio, Philip Allsopp, Pamela Magee, Alan Crozier, Ian Rowland, Chris Gill

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Fruit and vegetable consumption is associated at the population level with a protective effect against colorectal cancer. Phenolic compounds, especially abundant in berries, are of interest due to their putative anticancer activity. After consumption, however, phenolic compounds are subject to digestive conditions within the gastrointestinal tract that alter their structures and potentially their function. However, the majority of phenolic compounds are not efficiently absorbed in the small intestine and a substantial portion pass into the colon. We characterized berry extracts (raspberries, strawberries, blackcurrants) produced by in vitro-simulated upper intestinal tract digestion and subsequent fecal fermentation. These extracts and selected individual colonic metabolites were then evaluated for their putative anticancer activities using in vitro models of colorectal cancer, representing the key stages of initiation, promotion and invasion. Over a physiologically-relevant dose range (0-50 µg/ml gallic acid equivalents), the digested and fermented extracts demonstrated significant anti-genotoxic, anti-mutagenic and anti-invasive activity on colonocytes. This work indicates that phenolic compounds from berries undergo considerable structural modifications during their passage through the gastrointestinal tract but their breakdown products and metabolites retain biological activity and can modulate cellular processes associated with colon cancer.
LanguageEnglish
Pagese49740
JournalPLoS ONE
Volume7
Issue number11
Publication statusPublished - 2012

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Metabolites
Fermentation
small fruits
Digestion
phenolic compounds
Fruit
digestion
colorectal neoplasms
fermentation
Gallic Acid
extracts
Vegetables
Fruits
Bioactivity
gastrointestinal system
Gastrointestinal Tract
Colorectal Neoplasms
metabolites
Fragaria
black currants

Cite this

Brown, E. M., McDougall, G. J., Stewart, D., Pereira-Caro, G., González-Barrio, R., Allsopp, P., ... Gill, C. (2012). Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation. PLoS ONE, 7(11), e49740.
Brown, E.M ; McDougall, Gordon J ; Stewart, Derek ; Pereira-Caro, Gema ; González-Barrio, Rocio ; Allsopp, Philip ; Magee, Pamela ; Crozier, Alan ; Rowland, Ian ; Gill, Chris. / Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation. In: PLoS ONE. 2012 ; Vol. 7, No. 11. pp. e49740.
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Brown, EM, McDougall, GJ, Stewart, D, Pereira-Caro, G, González-Barrio, R, Allsopp, P, Magee, P, Crozier, A, Rowland, I & Gill, C 2012, 'Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation.', PLoS ONE, vol. 7, no. 11, pp. e49740.

Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation. / Brown, E.M; McDougall, Gordon J; Stewart, Derek; Pereira-Caro, Gema; González-Barrio, Rocio; Allsopp, Philip; Magee, Pamela; Crozier, Alan; Rowland, Ian; Gill, Chris.

In: PLoS ONE, Vol. 7, No. 11, 2012, p. e49740.

Research output: Contribution to journalArticle

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AU - Allsopp, Philip

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AU - Crozier, Alan

AU - Rowland, Ian

AU - Gill, Chris

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AB - Fruit and vegetable consumption is associated at the population level with a protective effect against colorectal cancer. Phenolic compounds, especially abundant in berries, are of interest due to their putative anticancer activity. After consumption, however, phenolic compounds are subject to digestive conditions within the gastrointestinal tract that alter their structures and potentially their function. However, the majority of phenolic compounds are not efficiently absorbed in the small intestine and a substantial portion pass into the colon. We characterized berry extracts (raspberries, strawberries, blackcurrants) produced by in vitro-simulated upper intestinal tract digestion and subsequent fecal fermentation. These extracts and selected individual colonic metabolites were then evaluated for their putative anticancer activities using in vitro models of colorectal cancer, representing the key stages of initiation, promotion and invasion. Over a physiologically-relevant dose range (0-50 µg/ml gallic acid equivalents), the digested and fermented extracts demonstrated significant anti-genotoxic, anti-mutagenic and anti-invasive activity on colonocytes. This work indicates that phenolic compounds from berries undergo considerable structural modifications during their passage through the gastrointestinal tract but their breakdown products and metabolites retain biological activity and can modulate cellular processes associated with colon cancer.

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Brown EM, McDougall GJ, Stewart D, Pereira-Caro G, González-Barrio R, Allsopp P et al. Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation. PLoS ONE. 2012;7(11):e49740.