Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis

Violetta Naughton (nee Lesniewska), T Ploszaj, W Barej, R Zabielski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The aim of the present study was to examine the contribution of the periodic pancreatic secretion (PPS) associated with a duodenal three-phased migrating myoelectric complex (MMC) to the intra-animal variability in protein electrophoretical profiles of pancreatic juice. The profiles of pancreatic juice proteins in the PPS trough and the PPS peak in the preprandial period, during milk feeding, and following intravenous and intraduodenal administration of CCK-8 were studied in six 2-week-old calves surgically fitted with pancreatic duct catheters, duodenal cannulas and 2 duodenal bipolar electrodes. Duodenal MMC recording served us with precise identification of PPS cycles. Juice protein samples, adjusted to 300 ng/ml, were applied to an automated SDS-polyacrylamide gel (12.5%) electrophoresis system. Gels were silver-stained, scanned and analysed for trace density (TD; band optical density x band height) by the computer. The separations varied in the number of bands, according to the phase of the PPS. In the PPS trough 17 protein bands were detected, and at the PPS peak, 21 bands. Moreover, within the detectable bands there were significant differences in the TD between the PPS trough and peak. Following feeding and CCK-8 infusion different numbers of protein bands were observed. In conclusion, by collecting pancreatic juice strictly in phase with its natural periodic secretion and analysing the juice proteins using a computerised approach, we have shown that pancreatic secretion is apparently not uniform. The differences concern the interdigestive as well as the prandial and CCK-8-stimulated composition of pancreatic juice.
LanguageEnglish
Title of host publicationBiology of the Pancreas in Growing Animals
EditorsStefan Grzegorz Pierzynowski, Romuald Zabielski
PublisherElsevier
Pages139-149
Volume28
ISBN (Print)0444502173
Publication statusPublished - 1999

Publication series

NameDEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES
PublisherElsevier Science Ltd

Fingerprint

Pancreatic Juice
Electrophoresis
Sincalide
Migrating Myoelectric Complexes
Proteins
Pancreatic Ducts
Silver
Intravenous Administration
Meals
Polyacrylamide Gel Electrophoresis
Electrodes
Milk
Catheters
Gels

Cite this

Naughton (nee Lesniewska), V., Ploszaj, T., Barej, W., & Zabielski, R. (1999). Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis. In S. G. Pierzynowski, & R. Zabielski (Eds.), Biology of the Pancreas in Growing Animals (Vol. 28, pp. 139-149). (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES). Elsevier.
Naughton (nee Lesniewska), Violetta ; Ploszaj, T ; Barej, W ; Zabielski, R. / Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis. Biology of the Pancreas in Growing Animals. editor / Stefan Grzegorz Pierzynowski ; Romuald Zabielski. Vol. 28 Elsevier, 1999. pp. 139-149 (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).
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abstract = "The aim of the present study was to examine the contribution of the periodic pancreatic secretion (PPS) associated with a duodenal three-phased migrating myoelectric complex (MMC) to the intra-animal variability in protein electrophoretical profiles of pancreatic juice. The profiles of pancreatic juice proteins in the PPS trough and the PPS peak in the preprandial period, during milk feeding, and following intravenous and intraduodenal administration of CCK-8 were studied in six 2-week-old calves surgically fitted with pancreatic duct catheters, duodenal cannulas and 2 duodenal bipolar electrodes. Duodenal MMC recording served us with precise identification of PPS cycles. Juice protein samples, adjusted to 300 ng/ml, were applied to an automated SDS-polyacrylamide gel (12.5{\%}) electrophoresis system. Gels were silver-stained, scanned and analysed for trace density (TD; band optical density x band height) by the computer. The separations varied in the number of bands, according to the phase of the PPS. In the PPS trough 17 protein bands were detected, and at the PPS peak, 21 bands. Moreover, within the detectable bands there were significant differences in the TD between the PPS trough and peak. Following feeding and CCK-8 infusion different numbers of protein bands were observed. In conclusion, by collecting pancreatic juice strictly in phase with its natural periodic secretion and analysing the juice proteins using a computerised approach, we have shown that pancreatic secretion is apparently not uniform. The differences concern the interdigestive as well as the prandial and CCK-8-stimulated composition of pancreatic juice.",
author = "{Naughton (nee Lesniewska)}, Violetta and T Ploszaj and W Barej and R Zabielski",
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Naughton (nee Lesniewska), V, Ploszaj, T, Barej, W & Zabielski, R 1999, Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis. in SG Pierzynowski & R Zabielski (eds), Biology of the Pancreas in Growing Animals. vol. 28, DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES, Elsevier, pp. 139-149.

Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis. / Naughton (nee Lesniewska), Violetta; Ploszaj, T; Barej, W; Zabielski, R.

Biology of the Pancreas in Growing Animals. ed. / Stefan Grzegorz Pierzynowski; Romuald Zabielski. Vol. 28 Elsevier, 1999. p. 139-149 (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis

AU - Naughton (nee Lesniewska), Violetta

AU - Ploszaj, T

AU - Barej, W

AU - Zabielski, R

N1 - Satellite Symposium on Biology of the Pancreas in Growing Animals at the 50th Annual Meeting of the European-Association-for-Animal-Production, ZURICH, SWITZERLAND, AUG, 1999

PY - 1999

Y1 - 1999

N2 - The aim of the present study was to examine the contribution of the periodic pancreatic secretion (PPS) associated with a duodenal three-phased migrating myoelectric complex (MMC) to the intra-animal variability in protein electrophoretical profiles of pancreatic juice. The profiles of pancreatic juice proteins in the PPS trough and the PPS peak in the preprandial period, during milk feeding, and following intravenous and intraduodenal administration of CCK-8 were studied in six 2-week-old calves surgically fitted with pancreatic duct catheters, duodenal cannulas and 2 duodenal bipolar electrodes. Duodenal MMC recording served us with precise identification of PPS cycles. Juice protein samples, adjusted to 300 ng/ml, were applied to an automated SDS-polyacrylamide gel (12.5%) electrophoresis system. Gels were silver-stained, scanned and analysed for trace density (TD; band optical density x band height) by the computer. The separations varied in the number of bands, according to the phase of the PPS. In the PPS trough 17 protein bands were detected, and at the PPS peak, 21 bands. Moreover, within the detectable bands there were significant differences in the TD between the PPS trough and peak. Following feeding and CCK-8 infusion different numbers of protein bands were observed. In conclusion, by collecting pancreatic juice strictly in phase with its natural periodic secretion and analysing the juice proteins using a computerised approach, we have shown that pancreatic secretion is apparently not uniform. The differences concern the interdigestive as well as the prandial and CCK-8-stimulated composition of pancreatic juice.

AB - The aim of the present study was to examine the contribution of the periodic pancreatic secretion (PPS) associated with a duodenal three-phased migrating myoelectric complex (MMC) to the intra-animal variability in protein electrophoretical profiles of pancreatic juice. The profiles of pancreatic juice proteins in the PPS trough and the PPS peak in the preprandial period, during milk feeding, and following intravenous and intraduodenal administration of CCK-8 were studied in six 2-week-old calves surgically fitted with pancreatic duct catheters, duodenal cannulas and 2 duodenal bipolar electrodes. Duodenal MMC recording served us with precise identification of PPS cycles. Juice protein samples, adjusted to 300 ng/ml, were applied to an automated SDS-polyacrylamide gel (12.5%) electrophoresis system. Gels were silver-stained, scanned and analysed for trace density (TD; band optical density x band height) by the computer. The separations varied in the number of bands, according to the phase of the PPS. In the PPS trough 17 protein bands were detected, and at the PPS peak, 21 bands. Moreover, within the detectable bands there were significant differences in the TD between the PPS trough and peak. Following feeding and CCK-8 infusion different numbers of protein bands were observed. In conclusion, by collecting pancreatic juice strictly in phase with its natural periodic secretion and analysing the juice proteins using a computerised approach, we have shown that pancreatic secretion is apparently not uniform. The differences concern the interdigestive as well as the prandial and CCK-8-stimulated composition of pancreatic juice.

M3 - Chapter

SN - 0444502173

VL - 28

T3 - DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES

SP - 139

EP - 149

BT - Biology of the Pancreas in Growing Animals

A2 - Pierzynowski, Stefan Grzegorz

A2 - Zabielski, Romuald

PB - Elsevier

ER -

Naughton (nee Lesniewska) V, Ploszaj T, Barej W, Zabielski R. Periodic pattern of secretion of pancreatic juice proteins in newborn calves demonstrated by SDS-page electrophoresis. In Pierzynowski SG, Zabielski R, editors, Biology of the Pancreas in Growing Animals. Vol. 28. Elsevier. 1999. p. 139-149. (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).