Peptidomic analysis of the host-defense peptides in skin secretions of Rana graecaprovidesinsightintophylogeneticrelationshipsamongEurasianRana species

Milena Mechkarska, J Kolodziejek, Vishal Musale, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, N Nowotny, JM Conlon

Research output: Contribution to journalArticle

Abstract

Peptidomic analysis of norepinephrine-stimulated skin secretions from the Greek stream frog Rana graeca Boulenger, 1891 led to the identification and structural characterization of a range of host-defense peptides. These comprised brevinin-1GRa, brevinin-1GRb and an N-terminally extended form of brevinin-1GRb, ranatuerin-2GR together with its oxidized form and (11–28) fragment, temporin-GRa, temporin-GRb and its nonamidated form, and a melittin-related peptide, MRP-GR and its (1–18) fragment. The most abundant peptide, MRP-GR significantly (P < 0.001) stimulated insulin release from BRIN-BD11 clonal β-cells at concentrations ≥0.1nM. Rana graeca (formerly Rana graeca graeca) and the morphologically similar Italian stream frog Rana italica Dubois, 1987 (formerly Rana graeca italica) were originally regarded as sub-species. However, the primary structures of the host defense peptides from both frogs support the claim based upon comparisons of the nucleotide sequences of S1 satellite DNA that R. graeca and R. italica are separate species. Cladistic analyses based upon the primary structures of the brevinin-1 and ranatuerin-2 peptides from Eurasian frogs indicate a close phylogenetic relationship between R. graeca and Rana latastei whereas R. italica is most closely related to Rana dalmatina.
LanguageEnglish
Article numberPart D 29
Pages228
Number of pages234
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume29
DOIs
Publication statusPublished - 27 Dec 2018

Fingerprint

Ranidae
Skin
Peptides
Anura
Melitten
Satellite DNA
Norepinephrine
Nucleotides
Insulin

Keywords

  • Host-defense peptide
  • Frog skin
  • brevinin-1
  • Ranatuerin-2
  • Temporin
  • Melittin-related peptide
  • Phylogenetics

Cite this

Mechkarska, Milena ; Kolodziejek, J ; Musale, Vishal ; Coquet, Laurent ; Leprince, Jérôme ; Jouenne, Thierry ; Nowotny, N ; Conlon, JM. / Peptidomic analysis of the host-defense peptides in skin secretions of Rana graecaprovidesinsightintophylogeneticrelationshipsamongEurasianRana species. 2018 ; Vol. 29. pp. 228.
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abstract = "Peptidomic analysis of norepinephrine-stimulated skin secretions from the Greek stream frog Rana graeca Boulenger, 1891 led to the identification and structural characterization of a range of host-defense peptides. These comprised brevinin-1GRa, brevinin-1GRb and an N-terminally extended form of brevinin-1GRb, ranatuerin-2GR together with its oxidized form and (11–28) fragment, temporin-GRa, temporin-GRb and its nonamidated form, and a melittin-related peptide, MRP-GR and its (1–18) fragment. The most abundant peptide, MRP-GR significantly (P < 0.001) stimulated insulin release from BRIN-BD11 clonal β-cells at concentrations ≥0.1nM. Rana graeca (formerly Rana graeca graeca) and the morphologically similar Italian stream frog Rana italica Dubois, 1987 (formerly Rana graeca italica) were originally regarded as sub-species. However, the primary structures of the host defense peptides from both frogs support the claim based upon comparisons of the nucleotide sequences of S1 satellite DNA that R. graeca and R. italica are separate species. Cladistic analyses based upon the primary structures of the brevinin-1 and ranatuerin-2 peptides from Eurasian frogs indicate a close phylogenetic relationship between R. graeca and Rana latastei whereas R. italica is most closely related to Rana dalmatina.",
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Peptidomic analysis of the host-defense peptides in skin secretions of Rana graecaprovidesinsightintophylogeneticrelationshipsamongEurasianRana species. / Mechkarska, Milena; Kolodziejek, J; Musale, Vishal; Coquet, Laurent; Leprince, Jérôme; Jouenne, Thierry; Nowotny, N; Conlon, JM.

Vol. 29, Part D 29, 27.12.2018, p. 228.

Research output: Contribution to journalArticle

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T1 - Peptidomic analysis of the host-defense peptides in skin secretions of Rana graecaprovidesinsightintophylogeneticrelationshipsamongEurasianRana species

AU - Mechkarska, Milena

AU - Kolodziejek, J

AU - Musale, Vishal

AU - Coquet, Laurent

AU - Leprince, Jérôme

AU - Jouenne, Thierry

AU - Nowotny, N

AU - Conlon, JM

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Y1 - 2018/12/27

N2 - Peptidomic analysis of norepinephrine-stimulated skin secretions from the Greek stream frog Rana graeca Boulenger, 1891 led to the identification and structural characterization of a range of host-defense peptides. These comprised brevinin-1GRa, brevinin-1GRb and an N-terminally extended form of brevinin-1GRb, ranatuerin-2GR together with its oxidized form and (11–28) fragment, temporin-GRa, temporin-GRb and its nonamidated form, and a melittin-related peptide, MRP-GR and its (1–18) fragment. The most abundant peptide, MRP-GR significantly (P < 0.001) stimulated insulin release from BRIN-BD11 clonal β-cells at concentrations ≥0.1nM. Rana graeca (formerly Rana graeca graeca) and the morphologically similar Italian stream frog Rana italica Dubois, 1987 (formerly Rana graeca italica) were originally regarded as sub-species. However, the primary structures of the host defense peptides from both frogs support the claim based upon comparisons of the nucleotide sequences of S1 satellite DNA that R. graeca and R. italica are separate species. Cladistic analyses based upon the primary structures of the brevinin-1 and ranatuerin-2 peptides from Eurasian frogs indicate a close phylogenetic relationship between R. graeca and Rana latastei whereas R. italica is most closely related to Rana dalmatina.

AB - Peptidomic analysis of norepinephrine-stimulated skin secretions from the Greek stream frog Rana graeca Boulenger, 1891 led to the identification and structural characterization of a range of host-defense peptides. These comprised brevinin-1GRa, brevinin-1GRb and an N-terminally extended form of brevinin-1GRb, ranatuerin-2GR together with its oxidized form and (11–28) fragment, temporin-GRa, temporin-GRb and its nonamidated form, and a melittin-related peptide, MRP-GR and its (1–18) fragment. The most abundant peptide, MRP-GR significantly (P < 0.001) stimulated insulin release from BRIN-BD11 clonal β-cells at concentrations ≥0.1nM. Rana graeca (formerly Rana graeca graeca) and the morphologically similar Italian stream frog Rana italica Dubois, 1987 (formerly Rana graeca italica) were originally regarded as sub-species. However, the primary structures of the host defense peptides from both frogs support the claim based upon comparisons of the nucleotide sequences of S1 satellite DNA that R. graeca and R. italica are separate species. Cladistic analyses based upon the primary structures of the brevinin-1 and ranatuerin-2 peptides from Eurasian frogs indicate a close phylogenetic relationship between R. graeca and Rana latastei whereas R. italica is most closely related to Rana dalmatina.

KW - Host-defense peptide

KW - Frog skin

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KW - Ranatuerin-2

KW - Temporin

KW - Melittin-related peptide

KW - Phylogenetics

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M3 - Article

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