Peptidomic analysis of the host-defense peptides in skin secretions of the Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae)

Milena Mechkarska, Laurent Coquet, Jérôme Leprince, Renoir Auguste, Thierry Jouenn, JM Conlon

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Peptidomic analysis (reversed-phase HPLC combined with MALDI-TOF mass spectrometry and automated Edman degradation) of norepinephrine-stimulated skin secretions from the Trinidadian leaf frog Phyllomedusa trinitatis Mertens 1926 led to the identification and structural characterization of 26 host-defense peptides. On the basis of amino acid sequence similarity, the peptides may be divided into the followings groups: dermaseptins with the conserved N-terminal region GLWSKIK (6 peptides), dermaseptins with the N-terminal region ALWKXXLK (5 peptides) dermaseptins with the conserved N-terminal region GLFKTLIKGAGKMLGHVAK (4 peptides), C-terminally α-amidated and non-amidated forms of the phylloseptins (9 peptides), phyllocaerulein, a peptide (GLVSGLLNSVTGLLGN LAGGGL) with structural similarity to the plasticins, and a putative antioxidant peptide (LTWKIPTRFCGVT). The primary structures of the peptides support the claim based upon morphological criteria that P. trinitatis and Phyllomedusa tarsius are very closely related phylogenetically but are probably not conspecific.
    LanguageEnglish
    Article numberxx
    Pagesxx
    Number of pages8
    JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
    Volumexx
    Issue numberxx
    Early online date30 Jun 2018
    DOIs
    Publication statusE-pub ahead of print - 30 Jun 2018

    Fingerprint

    Anura
    Skin
    Peptides
    Tarsiidae
    Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
    Mass spectrometry
    Amino Acid Sequence
    Mass Spectrometry
    Norepinephrine
    Antioxidants
    High Pressure Liquid Chromatography
    Amino Acids
    Degradation
    dermaseptin

    Keywords

    • host-defense peptide
    • antioxidant peptide
    • plasticin
    • phylloseptin
    • dermaseptin
    • Phyllomedusa
    • frog skin

    Cite this

    Mechkarska, Milena ; Coquet, Laurent ; Leprince, Jérôme ; Auguste, Renoir ; Jouenn, Thierry ; Conlon, JM. / Peptidomic analysis of the host-defense peptides in skin secretions of the Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae). 2018 ; Vol. xx, No. xx. pp. xx.
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    Peptidomic analysis of the host-defense peptides in skin secretions of the Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae). / Mechkarska, Milena; Coquet, Laurent; Leprince, Jérôme; Auguste, Renoir; Jouenn, Thierry; Conlon, JM.

    Vol. xx, No. xx, xx, 30.06.2018, p. xx.

    Research output: Contribution to journalArticle

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