Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)

Jay D. King; Milena Mechkarska; Mohammed A. Meetani; J. Michael Conlon

doi:10.1016/j.cbd.2013.07.001

Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)

Jay D. King, Milena Mechkarska, Mohammed A. Meetani, J. Michael Conlon

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Peptidomic analysis was used to compare the distribution of host-defense peptides in norepinephrine-stimulated skin secretions from Xenopus victorianus Ahl, 1924 (also described as the subspecies X. laevis victorianus) and Xenopus laevis sudanensis Perret, 1966 with the previously determined distributions in Xenopus laevis (Daudin, 1802) and Xenopus petersii Bocage, 1895. Peptides belonging to the magainin, peptide glycine-leucine-amide (PGLa), and caerulein precursor fragment (CPF) families were purified by reversed-phase HPLC and characterized by electrospray mass spectrometry. Magainin-P2, PGLa-P1, CPF-P1, CPF-P2, and CPF-P3 previously isolated from X. petersii and structurally different from orthologous peptides from X. laevis, were identified in X. victorianus and X. laevis sudanensis skin secretions whereas the corresponding X. laevis peptides were absent. Magainin-1, identical in X. petersii and X. laevis, was also identified in the secretions. Xenopsin-precursor fragment (XPF) peptides, absent from X. petersii but present in X. laevis skin secretions, were not identified in the X. victorianus and X. laevis sudanensis secretions. The data indicate that X. victorianus and X. laevis sudanensis are more closely related to X. petersii than to X. laevis and support separate species status. The study illustrates the value of analysis of host-defense peptides in the evaluation of taxonomic and phylogenetic relationships between closely related frog species.

Original language	English
Pages (from-to)	250-254
Number of pages	5
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/j.cbd.2013.07.001
Publication status	Published (in print/issue) - 2013

Bibliographical note

Funding Information:
This work was supported by a University Research Grant and a Faculty Support Grant ( NP/13/04 ) from the United Arab Emirates University . The authors thank Ben Evans, McMaster University, Hamilton, Canada for helpful discussions and Burley Lilley of Xenopus Express for help with sample collection.

Keywords

Frog skin
Host-defense peptide
Taxonomy
Xenopus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cbd.2013.07.001

Cite this

King, J. D., Mechkarska, M., Meetani, M. A., & Conlon, J. M. (2013). Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae). Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 8(3), 250-254. https://doi.org/10.1016/j.cbd.2013.07.001

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title = "Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)",

abstract = "Peptidomic analysis was used to compare the distribution of host-defense peptides in norepinephrine-stimulated skin secretions from Xenopus victorianus Ahl, 1924 (also described as the subspecies X. laevis victorianus) and Xenopus laevis sudanensis Perret, 1966 with the previously determined distributions in Xenopus laevis (Daudin, 1802) and Xenopus petersii Bocage, 1895. Peptides belonging to the magainin, peptide glycine-leucine-amide (PGLa), and caerulein precursor fragment (CPF) families were purified by reversed-phase HPLC and characterized by electrospray mass spectrometry. Magainin-P2, PGLa-P1, CPF-P1, CPF-P2, and CPF-P3 previously isolated from X. petersii and structurally different from orthologous peptides from X. laevis, were identified in X. victorianus and X. laevis sudanensis skin secretions whereas the corresponding X. laevis peptides were absent. Magainin-1, identical in X. petersii and X. laevis, was also identified in the secretions. Xenopsin-precursor fragment (XPF) peptides, absent from X. petersii but present in X. laevis skin secretions, were not identified in the X. victorianus and X. laevis sudanensis secretions. The data indicate that X. victorianus and X. laevis sudanensis are more closely related to X. petersii than to X. laevis and support separate species status. The study illustrates the value of analysis of host-defense peptides in the evaluation of taxonomic and phylogenetic relationships between closely related frog species.",

keywords = "Frog skin, Host-defense peptide, Taxonomy, Xenopus",

author = "King, {Jay D.} and Milena Mechkarska and Meetani, {Mohammed A.} and Conlon, {J. Michael}",

note = "Funding Information: This work was supported by a University Research Grant and a Faculty Support Grant ( NP/13/04 ) from the United Arab Emirates University . The authors thank Ben Evans, McMaster University, Hamilton, Canada for helpful discussions and Burley Lilley of Xenopus Express for help with sample collection.",

year = "2013",

doi = "10.1016/j.cbd.2013.07.001",

language = "English",

volume = "8",

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King, JD, Mechkarska, M, Meetani, MA & Conlon, JM 2013, 'Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)', Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, vol. 8, no. 3, pp. 250-254. https://doi.org/10.1016/j.cbd.2013.07.001

Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae). / King, Jay D.; Mechkarska, Milena; Meetani, Mohammed A. et al.
In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 8, No. 3, 2013, p. 250-254.

Research output: Contribution to journal › Article › peer-review

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T1 - Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)

AU - King, Jay D.

AU - Mechkarska, Milena

AU - Meetani, Mohammed A.

AU - Conlon, J. Michael

N1 - Funding Information: This work was supported by a University Research Grant and a Faculty Support Grant ( NP/13/04 ) from the United Arab Emirates University . The authors thank Ben Evans, McMaster University, Hamilton, Canada for helpful discussions and Burley Lilley of Xenopus Express for help with sample collection.

PY - 2013

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AB - Peptidomic analysis was used to compare the distribution of host-defense peptides in norepinephrine-stimulated skin secretions from Xenopus victorianus Ahl, 1924 (also described as the subspecies X. laevis victorianus) and Xenopus laevis sudanensis Perret, 1966 with the previously determined distributions in Xenopus laevis (Daudin, 1802) and Xenopus petersii Bocage, 1895. Peptides belonging to the magainin, peptide glycine-leucine-amide (PGLa), and caerulein precursor fragment (CPF) families were purified by reversed-phase HPLC and characterized by electrospray mass spectrometry. Magainin-P2, PGLa-P1, CPF-P1, CPF-P2, and CPF-P3 previously isolated from X. petersii and structurally different from orthologous peptides from X. laevis, were identified in X. victorianus and X. laevis sudanensis skin secretions whereas the corresponding X. laevis peptides were absent. Magainin-1, identical in X. petersii and X. laevis, was also identified in the secretions. Xenopsin-precursor fragment (XPF) peptides, absent from X. petersii but present in X. laevis skin secretions, were not identified in the X. victorianus and X. laevis sudanensis secretions. The data indicate that X. victorianus and X. laevis sudanensis are more closely related to X. petersii than to X. laevis and support separate species status. The study illustrates the value of analysis of host-defense peptides in the evaluation of taxonomic and phylogenetic relationships between closely related frog species.

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KW - Taxonomy

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SN - 1744-117X

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JO - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

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ER -

Peptidomic analysis of skin secretions provides insight into the taxonomic status of the African clawed frogs Xenopus victorianus and Xenopus laevis sudanensis (Pipidae)

Abstract

Bibliographical note

Keywords

UN SDGs

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