Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa

C Graham, A. E. Irvine, Stephen McClean, S. C. Richter, Peter Flatt, C. Shaw

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An octadecapeptide was isolated from the skin secretions of the dusky gopher frog (Rana sevosa) on the basis of histamine release from rat peritoneal mast cells. This peptide was purified to homogeneity by HPLC and found to have the following primary structure, YLKGCWTKSYPPKPCFSR, using both Edman degradation chemistry and peptide sequencing using high-resolution mass spectrometry (Q-TOF MS). The peptide, named peptide Tyrosine Arginine (pYR) shares 77.8% homology with peptide Leucine Arginine (pLR). The effects of the natural amidated peptide, non-amidated peptide and C-loop region of pYR on granulopoiesis and neutrophil apoptosis were investigated. All three analogues inhibited the early development of granulocyte macrophage colonies from bone marrow stem cells but did not induce apoptosis of the end stage granulocytes, the mature neutrophil. Thus, pYR is a novel member of an important and emerging new class of amphibian peptides with hemopoietic actions.
Original languageEnglish
Pages (from-to)737-743
JournalPeptides
Volume26
Issue number5
Publication statusPublished - May 2005

Keywords

  • Amino Acid Sequence
  • Amphibian Proteins/chemistry/isolation &amp
  • purification/pharmacology
  • Animals
  • Colony-Forming Units Assay
  • Granulocytes/drug effects
  • Histamine Release/drug effects
  • Immunologic Factors/chemistry/isolation &amp
  • Macrophages/drug effects
  • Male
  • Mast Cells/drug effects
  • Molecular Sequence Data
  • Peptides/chemistry/isolation &amp
  • purification
  • Ranidae/metabolism
  • Rats
  • Skin/secretion

Fingerprint Dive into the research topics of 'Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa'. Together they form a unique fingerprint.

  • Cite this