Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor

Isabelle Lihrmann; Jean Chris Tophe Plaquevent; Rianne Raijmakers; Herve Tostivint; Marie Christine Tonon; Hubert Vaudry; J. Michael Conlon

doi:10.1163/156854295X00492

Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor

Isabelle Lihrmann, Jean Chris Tophe Plaquevent, Rianne Raijmakers, Herve Tostivint, Marie Christine Tonon, Hubert Vaudry, J. Michael Conlon

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.

Original language	English
Pages (from-to)	18-21
Number of pages	4
Journal	Netherlands Journal of Zoology
Volume	45
Issue number	1-2
DOIs	https://doi.org/10.1163/156854295X00492
Publication status	Published (in print/issue) - 1994

Keywords

benzodiazepine-receptor ligands
evolution
glial cclls
nucleotide sequence
peptide microsequcncing
RT-PCR

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10.1163/156854295X00492

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title = "Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor",

abstract = "Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.",

keywords = "benzodiazepine-receptor ligands, evolution, glial cclls, nucleotide sequence, peptide microsequcncing, RT-PCR",

author = "Isabelle Lihrmann and {Tophe Plaquevent}, {Jean Chris} and Rianne Raijmakers and Herve Tostivint and Tonon, {Marie Christine} and Hubert Vaudry and {Michael Conlon}, J.",

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doi = "10.1163/156854295X00492",

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T1 - Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor

AU - Lihrmann, Isabelle

AU - Tophe Plaquevent, Jean Chris

AU - Raijmakers, Rianne

AU - Tostivint, Herve

AU - Tonon, Marie Christine

AU - Vaudry, Hubert

AU - Michael Conlon, J.

PY - 1994

Y1 - 1994

N2 - Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.

AB - Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.

KW - benzodiazepine-receptor ligands

KW - evolution

KW - glial cclls

KW - nucleotide sequence

KW - peptide microsequcncing

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=0004396169&partnerID=8YFLogxK

U2 - 10.1163/156854295X00492

DO - 10.1163/156854295X00492

M3 - Article

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VL - 45

SP - 18

EP - 21

JO - Netherlands Journal of Zoology

JF - Netherlands Journal of Zoology

IS - 1-2

ER -

Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor

Abstract

Keywords

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