Patterned cell culture substrates created by hot embossing of tissue culture treated polystyrene

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Abstract

Patterning materials such that they elicit a different cell response in different regions would have significant implications in fields such as implantable biomaterials, in vitro cell culture and tissue engineering and regenerative medicine. Moreover, the ability to pattern polymers using inexpensive, currently available processes, without the need for adding proteins or other biochemical agents could lead to new opportunities in biomaterials research. The research reported here demonstrates that bycombining the plasma surface treatments used to create commercial grade tissue culture treated polystyrene, with controlled hot embossing processes, that distinct regions can be created on a substrate that result in spatial control of endothelial cell adhesion and proliferation. As well as the topographical changes that result from hot embossing, significant changes in surface chemistry and wettability have been observed and characterised and the resultant effects on endothelial cell responses evaluated. Byspatially controlling endothelial cell adhesion, proliferation and subsequent angiogenesis, the processes outlined here have the potential to be used to create a range of different substrates, with applications in the development of assays for high throughput screening, the patterning of implantable biomaterials or the development of smart scaffolds for tissue engineering.
LanguageEnglish
JournalJournal of Materials Science: Materials in Medicine
Volumeonline
DOIs
Publication statusPublished - 2013

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Tissue culture
Polystyrenes
Endothelial cells
Biocompatible Materials
Cell culture
Biomaterials
Endothelial Cells
Cell Culture Techniques
Cell adhesion
Tissue Engineering
Tissue engineering
Cell Adhesion
Substrates
Cell Proliferation
High-Throughput Screening Assays
Cell Engineering
Wettability
Regenerative Medicine
Cell proliferation
Scaffolds (biology)

Cite this

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title = "Patterned cell culture substrates created by hot embossing of tissue culture treated polystyrene",
abstract = "Patterning materials such that they elicit a different cell response in different regions would have significant implications in fields such as implantable biomaterials, in vitro cell culture and tissue engineering and regenerative medicine. Moreover, the ability to pattern polymers using inexpensive, currently available processes, without the need for adding proteins or other biochemical agents could lead to new opportunities in biomaterials research. The research reported here demonstrates that bycombining the plasma surface treatments used to create commercial grade tissue culture treated polystyrene, with controlled hot embossing processes, that distinct regions can be created on a substrate that result in spatial control of endothelial cell adhesion and proliferation. As well as the topographical changes that result from hot embossing, significant changes in surface chemistry and wettability have been observed and characterised and the resultant effects on endothelial cell responses evaluated. Byspatially controlling endothelial cell adhesion, proliferation and subsequent angiogenesis, the processes outlined here have the potential to be used to create a range of different substrates, with applications in the development of assays for high throughput screening, the patterning of implantable biomaterials or the development of smart scaffolds for tissue engineering.",
author = "Alan Brown and GA Burke and BJ Meenan",
year = "2013",
doi = "10.1007/s10856-013-5011-5",
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TY - JOUR

T1 - Patterned cell culture substrates created by hot embossing of tissue culture treated polystyrene

AU - Brown, Alan

AU - Burke, GA

AU - Meenan, BJ

PY - 2013

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N2 - Patterning materials such that they elicit a different cell response in different regions would have significant implications in fields such as implantable biomaterials, in vitro cell culture and tissue engineering and regenerative medicine. Moreover, the ability to pattern polymers using inexpensive, currently available processes, without the need for adding proteins or other biochemical agents could lead to new opportunities in biomaterials research. The research reported here demonstrates that bycombining the plasma surface treatments used to create commercial grade tissue culture treated polystyrene, with controlled hot embossing processes, that distinct regions can be created on a substrate that result in spatial control of endothelial cell adhesion and proliferation. As well as the topographical changes that result from hot embossing, significant changes in surface chemistry and wettability have been observed and characterised and the resultant effects on endothelial cell responses evaluated. Byspatially controlling endothelial cell adhesion, proliferation and subsequent angiogenesis, the processes outlined here have the potential to be used to create a range of different substrates, with applications in the development of assays for high throughput screening, the patterning of implantable biomaterials or the development of smart scaffolds for tissue engineering.

AB - Patterning materials such that they elicit a different cell response in different regions would have significant implications in fields such as implantable biomaterials, in vitro cell culture and tissue engineering and regenerative medicine. Moreover, the ability to pattern polymers using inexpensive, currently available processes, without the need for adding proteins or other biochemical agents could lead to new opportunities in biomaterials research. The research reported here demonstrates that bycombining the plasma surface treatments used to create commercial grade tissue culture treated polystyrene, with controlled hot embossing processes, that distinct regions can be created on a substrate that result in spatial control of endothelial cell adhesion and proliferation. As well as the topographical changes that result from hot embossing, significant changes in surface chemistry and wettability have been observed and characterised and the resultant effects on endothelial cell responses evaluated. Byspatially controlling endothelial cell adhesion, proliferation and subsequent angiogenesis, the processes outlined here have the potential to be used to create a range of different substrates, with applications in the development of assays for high throughput screening, the patterning of implantable biomaterials or the development of smart scaffolds for tissue engineering.

U2 - 10.1007/s10856-013-5011-5

DO - 10.1007/s10856-013-5011-5

M3 - Article

VL - online

JO - Journal of Materials Science: Materials in Medicine

T2 - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

SN - 0957-4530

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