PATERNALLY DERIVED H19 IS DIFFERENTIALLY EXPRESSED IN MALIGNANT AND NONMALIGNANT TROPHOBLAST

Colum Walsh, SJ MILLER, F FLAM, RA FISHER, R OHLSSON

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Abstract

The paternal allele of the H19 gene has been shown to be transcriptionally inactive in the developing human embryo. Using reverse transcription PCR and RNase protection assays, we demonstrate that expression of H19 is predominantly, but not exclusively, from the maternal allele in the human placenta. In situ hybridization analysis shows strong expression of the H19 gene in eight complete hydatidiform moles, hyperplastic tissues consisting of trophoblasts which contain only paternally derived genetic material, indicating that H19 is not functionally imprinted in this tissue. H19, a putative growth suppressor, is oppositely imprinted to the neighboring insulin-like growth factor II (IGF2) gene and an up-regulation of IGF2 expression has been linked previously to a down-regulation of H19 expression in the progression to Wilms' tumor. Two cases of complete hydatidiform mole which progressed to choriocarcinoma show high levels of expression of both H19 and IGF2. The choriocarcinomas which developed from these complete hydatiform moles showed similar expression of IGF2 but a decreased number of H19-positive cells, which may reflect selection for cells expressing IGF2 and against those expressing H19 in this tissue.
LanguageEnglish
Pages1111-1116
JournalCancer Research
Volume55
Issue number5
Publication statusPublished - Mar 1995

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Trophoblasts
Hydatidiform Mole
Choriocarcinoma
Alleles
Genes
Insulin-Like Growth Factor II
Wilms Tumor
Ribonucleases
Placenta
Reverse Transcription
In Situ Hybridization
Up-Regulation
Down-Regulation
Embryonic Structures
Mothers
Gene Expression
Polymerase Chain Reaction
Growth

Cite this

Walsh, C., MILLER, SJ., FLAM, F., FISHER, RA., & OHLSSON, R. (1995). PATERNALLY DERIVED H19 IS DIFFERENTIALLY EXPRESSED IN MALIGNANT AND NONMALIGNANT TROPHOBLAST. Cancer Research, 55(5), 1111-1116.
Walsh, Colum ; MILLER, SJ ; FLAM, F ; FISHER, RA ; OHLSSON, R. / PATERNALLY DERIVED H19 IS DIFFERENTIALLY EXPRESSED IN MALIGNANT AND NONMALIGNANT TROPHOBLAST. In: Cancer Research. 1995 ; Vol. 55, No. 5. pp. 1111-1116.
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abstract = "The paternal allele of the H19 gene has been shown to be transcriptionally inactive in the developing human embryo. Using reverse transcription PCR and RNase protection assays, we demonstrate that expression of H19 is predominantly, but not exclusively, from the maternal allele in the human placenta. In situ hybridization analysis shows strong expression of the H19 gene in eight complete hydatidiform moles, hyperplastic tissues consisting of trophoblasts which contain only paternally derived genetic material, indicating that H19 is not functionally imprinted in this tissue. H19, a putative growth suppressor, is oppositely imprinted to the neighboring insulin-like growth factor II (IGF2) gene and an up-regulation of IGF2 expression has been linked previously to a down-regulation of H19 expression in the progression to Wilms' tumor. Two cases of complete hydatidiform mole which progressed to choriocarcinoma show high levels of expression of both H19 and IGF2. The choriocarcinomas which developed from these complete hydatiform moles showed similar expression of IGF2 but a decreased number of H19-positive cells, which may reflect selection for cells expressing IGF2 and against those expressing H19 in this tissue.",
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Walsh, C, MILLER, SJ, FLAM, F, FISHER, RA & OHLSSON, R 1995, 'PATERNALLY DERIVED H19 IS DIFFERENTIALLY EXPRESSED IN MALIGNANT AND NONMALIGNANT TROPHOBLAST', Cancer Research, vol. 55, no. 5, pp. 1111-1116.

PATERNALLY DERIVED H19 IS DIFFERENTIALLY EXPRESSED IN MALIGNANT AND NONMALIGNANT TROPHOBLAST. / Walsh, Colum; MILLER, SJ; FLAM, F; FISHER, RA; OHLSSON, R.

In: Cancer Research, Vol. 55, No. 5, 03.1995, p. 1111-1116.

Research output: Contribution to journalArticle

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N2 - The paternal allele of the H19 gene has been shown to be transcriptionally inactive in the developing human embryo. Using reverse transcription PCR and RNase protection assays, we demonstrate that expression of H19 is predominantly, but not exclusively, from the maternal allele in the human placenta. In situ hybridization analysis shows strong expression of the H19 gene in eight complete hydatidiform moles, hyperplastic tissues consisting of trophoblasts which contain only paternally derived genetic material, indicating that H19 is not functionally imprinted in this tissue. H19, a putative growth suppressor, is oppositely imprinted to the neighboring insulin-like growth factor II (IGF2) gene and an up-regulation of IGF2 expression has been linked previously to a down-regulation of H19 expression in the progression to Wilms' tumor. Two cases of complete hydatidiform mole which progressed to choriocarcinoma show high levels of expression of both H19 and IGF2. The choriocarcinomas which developed from these complete hydatiform moles showed similar expression of IGF2 but a decreased number of H19-positive cells, which may reflect selection for cells expressing IGF2 and against those expressing H19 in this tissue.

AB - The paternal allele of the H19 gene has been shown to be transcriptionally inactive in the developing human embryo. Using reverse transcription PCR and RNase protection assays, we demonstrate that expression of H19 is predominantly, but not exclusively, from the maternal allele in the human placenta. In situ hybridization analysis shows strong expression of the H19 gene in eight complete hydatidiform moles, hyperplastic tissues consisting of trophoblasts which contain only paternally derived genetic material, indicating that H19 is not functionally imprinted in this tissue. H19, a putative growth suppressor, is oppositely imprinted to the neighboring insulin-like growth factor II (IGF2) gene and an up-regulation of IGF2 expression has been linked previously to a down-regulation of H19 expression in the progression to Wilms' tumor. Two cases of complete hydatidiform mole which progressed to choriocarcinoma show high levels of expression of both H19 and IGF2. The choriocarcinomas which developed from these complete hydatiform moles showed similar expression of IGF2 but a decreased number of H19-positive cells, which may reflect selection for cells expressing IGF2 and against those expressing H19 in this tissue.

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