Paper-based electrode assemble for impedimetric detection of miRNA

E Eksin, H Torul, E Yarali, U Tamer, P Papakonstantinou, A Erdem

Research output: Contribution to journalArticlepeer-review

Abstract

In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.
Original languageEnglish
Article number122043
Pages (from-to)122043
Number of pages6
JournalTalanta
Volume225
Early online date30 Dec 2020
DOIs
Publication statusE-pub ahead of print - 30 Dec 2020

Keywords

  • miRNA
  • Paper based sensor
  • micro-RNA
  • impedimetric sensor
  • gold electrodeposition
  • gold nanoparticles
  • Gold nanoparticles
  • microRNA
  • Paper based electrode
  • Electrochemical impedance spectroscopy

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