Paper-based electrode assemble for impedimetric detection of miRNA

E Eksin; H Torul; E Yarali; U Tamer; P Papakonstantinou; A Erdem

doi:10.1016/j.talanta.2020.122043

Paper-based electrode assemble for impedimetric detection of miRNA

E Eksin, H Torul, E Yarali, U Tamer, P Papakonstantinou, A Erdem

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Abstract

In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.

Original language	English
Article number	122043
Pages (from-to)	122043
Number of pages	6
Journal	Talanta
Volume	225
Early online date	30 Dec 2020
DOIs	https://doi.org/10.1016/j.talanta.2020.122043
Publication status	Published (in print/issue) - 1 Apr 2021

Bibliographical note

Funding Information:
This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.

Publisher Copyright:
© 2020 Elsevier B.V.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Funding Information:
This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.

Funding Information:
This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.

Publisher Copyright:
© 2020 Elsevier B.V.

Copyright © 2020 Elsevier B.V. All rights reserved.

Keywords

miRNA
Paper based sensor
micro-RNA
impedimetric sensor
gold electrodeposition
gold nanoparticles
Gold nanoparticles
microRNA
Paper based electrode
Electrochemical impedance spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.talanta.2020.122043

mi-RNA based paper sensor-Author Accepted Manuscript, 605 KBLicence: CC BY-NC-ND
miRNA paper based sensor supporting information

Cite this

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title = "Paper-based electrode assemble for impedimetric detection of miRNA",

abstract = "In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS. ",

keywords = "miRNA, Paper based sensor, micro-RNA, impedimetric sensor, gold electrodeposition, gold nanoparticles, Gold nanoparticles, microRNA, Paper based electrode, Electrochemical impedance spectroscopy",

author = "E Eksin and H Torul and E Yarali and U Tamer and P Papakonstantinou and A Erdem",

note = "Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved. Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright {\textcopyright} 2020 Elsevier B.V. All rights reserved.",

year = "2021",

month = apr,

day = "1",

doi = "10.1016/j.talanta.2020.122043",

language = "English",

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AU - Torul, H

AU - Yarali, E

AU - Tamer, U

AU - Papakonstantinou, P

AU - Erdem, A

N1 - Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved. Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Funding Information: This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. Publisher Copyright: © 2020 Elsevier B.V. Copyright © 2020 Elsevier B.V. All rights reserved.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.

AB - In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.

KW - miRNA

KW - Paper based sensor

KW - micro-RNA

KW - impedimetric sensor

KW - gold electrodeposition

KW - gold nanoparticles

KW - Gold nanoparticles

KW - microRNA

KW - Paper based electrode

KW - Electrochemical impedance spectroscopy

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DO - 10.1016/j.talanta.2020.122043

M3 - Article

C2 - 33592766

SN - 0039-9140

VL - 225

SP - 122043

JO - Talanta

JF - Talanta

M1 - 122043

ER -

Paper-based electrode assemble for impedimetric detection of miRNA

Abstract

Bibliographical note

Keywords

UN SDGs

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