Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS2) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 µL sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were found to be 12.0 and 25.7 nM for AuNPs/RGO and 51.6 and 59.6 nM for AuNPs/MoS2 sensors in the case of perfectly matched probe-target hybrids. These biosensors were found to be selective enough to distinguish the target miRNA in the presence of single-base mismatch miRNA or noncomplementary miRNA sequences.
Bibliographical noteFunding Information:
This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702), the British Council (Newton Fund, Institutional Links, Ref: 216182787) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- differential pulse voltammetry
- paper-based biosensor
- molybdenum disulfide nanosheets
- gold nanohybrids
- reduced graphene oxide
- Molybdenum disulfide nanosheets
- Paper-based biosensor
- Gold nanohybrids
- Reduced graphene oxide
- Differential pulse voltammetry