Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS

Hilal Torul, Ece Yarali, Ece Eksin, Abhijit Ganguly, John Benson, Ugur Tamer, Pagona Papakonstantinou, Arzum Erdem

Research output: Contribution to journalArticlepeer-review

Abstract

Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS ) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 µL sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were found to be 12.0 and 25.7 nM for AuNPs/RGO and 51.6 and 59.6 nM for AuNPs/MoS sensors in the case of perfectly matched probe-target hybrids. These biosensors were found to be selective enough to distinguish the target miRNA in the presence of single-base mismatch miRNA or noncomplementary miRNA sequences.
Original languageEnglish
JournalBiosensors
Volume11
Issue number7
Early online date13 Jul 2021
DOIs
Publication statusE-pub ahead of print - 13 Jul 2021

Keywords

  • differential pulse voltammetry
  • paper-based biosensor
  • molybdenum disulfide nanosheets
  • microRNA
  • gold nanohybrids
  • reduced graphene oxide

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