Pancreatic exocrine secretions as a source of luminal polyamines in pigs

S Loret, P Brolet, S Pierzynowski, I Gouders, M Klimek, V Danielson, A Rosted, Violetta Naughton, G Dandrifosse

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The goal of the present study was twofold: (1) to detect the possible storage of dietary polyamines (PAs) in various tissues and (2) to investigate the role of dietary PAs in the differentiation of the pig intestinal epithelium. A first experimental series was designed to assess the accumulation of either milk PAs (mostly spermidine) or orally administered spermine (SPM) in piglet red blood cells (RBCs) and plasma, a preliminary stage in their distribution to growing and storage organs. Though PA concentrations of piglet RBCs and plasma were generally significantly higher than their sow counterparts, our experimental conditions failed to demonstrate that this increase could stem from ingested PAs. A second experimental series dealt with the determination of disaccharidase specific activities in proximal and distal parts of piglet gut on the 26th and 29th days after birth (preweaning time). In agreement with observations made previously on rat pups, we observed an increase in maltase specific activity (SA) at the end of the suckling period (the observed increase in sucrase SA was not significant). However, orally administered SPM did not affect this activity. Compared to the constant protein concentrations observed in both parts of the gut, the pancreatic protein content decreased sharply between the 26th and 29th postnatal days. At the same time pancreatic concentrations of spermidine (SPD) also decreased, suggesting that some pancreatic PAs were released as the organ secreted its proteins. In accordance with this hypothesis, we recorded SPM and SPD in pancreatic juice. The increases in PA concentrations seemed to follow the protein secretion pattern (i.e. PA concentrations reached a maximal value when the protein concentration was highest). The presence of PAs in pancreatic juice could be indicative of a control mechanism exerted by the pancreas on PA-induced growth and differentiation of porcine intestinal epithelium.
LanguageEnglish
Pages301-308
JournalExperimental Physiology
Volume85
Issue number3
Publication statusPublished - May 2000

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polyamines
secretion
swine
spermine
spermidine
pancreatic juice
piglets
intestinal mucosa
blood plasma
erythrocytes
digestive system
sucrose alpha-glucosidase
storage organs
protein secretion
protein value
alpha-glucosidase
suckling
pancreas
pups
sows

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Loret, S., Brolet, P., Pierzynowski, S., Gouders, I., Klimek, M., Danielson, V., ... Dandrifosse, G. (2000). Pancreatic exocrine secretions as a source of luminal polyamines in pigs. Experimental Physiology, 85(3), 301-308.
Loret, S ; Brolet, P ; Pierzynowski, S ; Gouders, I ; Klimek, M ; Danielson, V ; Rosted, A ; Naughton, Violetta ; Dandrifosse, G. / Pancreatic exocrine secretions as a source of luminal polyamines in pigs. In: Experimental Physiology. 2000 ; Vol. 85, No. 3. pp. 301-308.
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Loret, S, Brolet, P, Pierzynowski, S, Gouders, I, Klimek, M, Danielson, V, Rosted, A, Naughton, V & Dandrifosse, G 2000, 'Pancreatic exocrine secretions as a source of luminal polyamines in pigs', Experimental Physiology, vol. 85, no. 3, pp. 301-308.

Pancreatic exocrine secretions as a source of luminal polyamines in pigs. / Loret, S; Brolet, P; Pierzynowski, S; Gouders, I; Klimek, M; Danielson, V; Rosted, A; Naughton, Violetta; Dandrifosse, G.

In: Experimental Physiology, Vol. 85, No. 3, 05.2000, p. 301-308.

Research output: Contribution to journalArticle

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T1 - Pancreatic exocrine secretions as a source of luminal polyamines in pigs

AU - Loret, S

AU - Brolet, P

AU - Pierzynowski, S

AU - Gouders, I

AU - Klimek, M

AU - Danielson, V

AU - Rosted, A

AU - Naughton, Violetta

AU - Dandrifosse, G

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N2 - The goal of the present study was twofold: (1) to detect the possible storage of dietary polyamines (PAs) in various tissues and (2) to investigate the role of dietary PAs in the differentiation of the pig intestinal epithelium. A first experimental series was designed to assess the accumulation of either milk PAs (mostly spermidine) or orally administered spermine (SPM) in piglet red blood cells (RBCs) and plasma, a preliminary stage in their distribution to growing and storage organs. Though PA concentrations of piglet RBCs and plasma were generally significantly higher than their sow counterparts, our experimental conditions failed to demonstrate that this increase could stem from ingested PAs. A second experimental series dealt with the determination of disaccharidase specific activities in proximal and distal parts of piglet gut on the 26th and 29th days after birth (preweaning time). In agreement with observations made previously on rat pups, we observed an increase in maltase specific activity (SA) at the end of the suckling period (the observed increase in sucrase SA was not significant). However, orally administered SPM did not affect this activity. Compared to the constant protein concentrations observed in both parts of the gut, the pancreatic protein content decreased sharply between the 26th and 29th postnatal days. At the same time pancreatic concentrations of spermidine (SPD) also decreased, suggesting that some pancreatic PAs were released as the organ secreted its proteins. In accordance with this hypothesis, we recorded SPM and SPD in pancreatic juice. The increases in PA concentrations seemed to follow the protein secretion pattern (i.e. PA concentrations reached a maximal value when the protein concentration was highest). The presence of PAs in pancreatic juice could be indicative of a control mechanism exerted by the pancreas on PA-induced growth and differentiation of porcine intestinal epithelium.

AB - The goal of the present study was twofold: (1) to detect the possible storage of dietary polyamines (PAs) in various tissues and (2) to investigate the role of dietary PAs in the differentiation of the pig intestinal epithelium. A first experimental series was designed to assess the accumulation of either milk PAs (mostly spermidine) or orally administered spermine (SPM) in piglet red blood cells (RBCs) and plasma, a preliminary stage in their distribution to growing and storage organs. Though PA concentrations of piglet RBCs and plasma were generally significantly higher than their sow counterparts, our experimental conditions failed to demonstrate that this increase could stem from ingested PAs. A second experimental series dealt with the determination of disaccharidase specific activities in proximal and distal parts of piglet gut on the 26th and 29th days after birth (preweaning time). In agreement with observations made previously on rat pups, we observed an increase in maltase specific activity (SA) at the end of the suckling period (the observed increase in sucrase SA was not significant). However, orally administered SPM did not affect this activity. Compared to the constant protein concentrations observed in both parts of the gut, the pancreatic protein content decreased sharply between the 26th and 29th postnatal days. At the same time pancreatic concentrations of spermidine (SPD) also decreased, suggesting that some pancreatic PAs were released as the organ secreted its proteins. In accordance with this hypothesis, we recorded SPM and SPD in pancreatic juice. The increases in PA concentrations seemed to follow the protein secretion pattern (i.e. PA concentrations reached a maximal value when the protein concentration was highest). The presence of PAs in pancreatic juice could be indicative of a control mechanism exerted by the pancreas on PA-induced growth and differentiation of porcine intestinal epithelium.

M3 - Article

VL - 85

SP - 301

EP - 308

JO - Experimental Physiology

T2 - Experimental Physiology

JF - Experimental Physiology

SN - 0958-0670

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Loret S, Brolet P, Pierzynowski S, Gouders I, Klimek M, Danielson V et al. Pancreatic exocrine secretions as a source of luminal polyamines in pigs. Experimental Physiology. 2000 May;85(3):301-308.