Oxytocin is present in islets and plays a role in beta-cell function and survival

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Oxytocin is associated mainly with modulating reproductive function. However, studies suggest that oxytocin also plays a role in endocrine pancreatic function. In the present study, islet expression of oxytocin and its related receptor was confirmed in mouse islets as well as cultured rodent and human beta-cells. Oxytocin significantly stimulated glucose-induced insulin secretion from isolated mouse islets. Similar insulinotropic actions were also observed in rodent BRIN BD11 and human 1.1B4 beta-cells. Positive effects of oxytocin on insulin secretion were almost fully annulled by the oxytocin receptor antagonist, atosiban. In terms of mechanism of insulin secretory action, oxytocin had no effect on beta-cell membrane potential or cAMP generation, but did augment intracellular calcium concentrations. In vivo administration of oxytocin to mice significantly reduced overall blood glucose levels and increased plasma insulin concentrations in response to a glucose challenge. Oxytocin also had a modest, but significant, appetite suppressive effect. As expected, streptozotocin diabetic mice had marked loss of beta-cell area accompanied by increases in alpha-cell area, whilst hydrocortisone treatment increased beta-cell and overall islet areas. Both mouse models of diabetes presented with dramatically decreased percentage islet oxytocin co-localisation with insulin and increased co-localisation with glucagon. More detailed studies in cultured beta-cell lines revealed direct positive effects of oxytocin on beta-cell proliferation and protection against apoptosis. Together, these data highlight a potentially important role of islet-derived oxytocin and related receptor signalling pathways on the modulation of beta-cell function and survival.
LanguageEnglish
Pages260-268
JournalPeptides
Volume100
Early online date3 Feb 2018
DOIs
Publication statusE-pub ahead of print - 3 Feb 2018

Fingerprint

Oxytocin
Cell Survival
Insulin
Oxytocin Receptors
Rodentia
Glucose
Cytoprotection
Appetite
Streptozocin
Glucagon
Cell proliferation
Islets of Langerhans
Cell membranes
Membrane Potentials
Medical problems
Hydrocortisone
Blood Glucose
Cultured Cells
Cell Proliferation
Cell Membrane

Keywords

  • Beta-cell
  • islets
  • oxytocin
  • oxytocin receptor
  • insulin secretion
  • diabetes
  • proliferation
  • apoptosis

Cite this

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abstract = "Oxytocin is associated mainly with modulating reproductive function. However, studies suggest that oxytocin also plays a role in endocrine pancreatic function. In the present study, islet expression of oxytocin and its related receptor was confirmed in mouse islets as well as cultured rodent and human beta-cells. Oxytocin significantly stimulated glucose-induced insulin secretion from isolated mouse islets. Similar insulinotropic actions were also observed in rodent BRIN BD11 and human 1.1B4 beta-cells. Positive effects of oxytocin on insulin secretion were almost fully annulled by the oxytocin receptor antagonist, atosiban. In terms of mechanism of insulin secretory action, oxytocin had no effect on beta-cell membrane potential or cAMP generation, but did augment intracellular calcium concentrations. In vivo administration of oxytocin to mice significantly reduced overall blood glucose levels and increased plasma insulin concentrations in response to a glucose challenge. Oxytocin also had a modest, but significant, appetite suppressive effect. As expected, streptozotocin diabetic mice had marked loss of beta-cell area accompanied by increases in alpha-cell area, whilst hydrocortisone treatment increased beta-cell and overall islet areas. Both mouse models of diabetes presented with dramatically decreased percentage islet oxytocin co-localisation with insulin and increased co-localisation with glucagon. More detailed studies in cultured beta-cell lines revealed direct positive effects of oxytocin on beta-cell proliferation and protection against apoptosis. Together, these data highlight a potentially important role of islet-derived oxytocin and related receptor signalling pathways on the modulation of beta-cell function and survival.",
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Oxytocin is present in islets and plays a role in beta-cell function and survival. / Mohan, S; Khan, D; Moffett, Charlotte; Irwin, Nigel; Flatt, Peter.

In: Peptides, Vol. 100, 03.02.2018, p. 260-268.

Research output: Contribution to journalArticle

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AU - Khan, D

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AU - Irwin, Nigel

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