Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production

Iza Radecka, Victor Irorere, Guozhan Jiang, David Hill, Craig Williams, Grazyna Adamus, Michal Kwiecień, Adam A Marek, Jan Zawadiak, Brian Johnston, Marek Kowalczuk, Naozumi Teramoto (Editor)

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.
LanguageEnglish
JournalMaterials
Volume9
Issue number5
DOIs
Publication statusPublished - May 2016

Fingerprint

Polyhydroxyalkanoates
Waxes
Polyethylene
Polyethylenes
Carbon
3-Hydroxybutyric Acid
Bacteria
Electrospray ionization
Biopolymers
Gel permeation chromatography
Oxidants
Mass spectrometry
Thermogravimetric analysis
Differential scanning calorimetry
Biomass
Salts
Nuclear magnetic resonance
Oxygen
tryptones
Air

Cite this

Radecka, I., Irorere, V., Jiang, G., Hill, D., Williams, C., Adamus, G., ... Teramoto, N. (Ed.) (2016). Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production. Materials, 9(5). https://doi.org/10.3390/ma9050367
Radecka, Iza ; Irorere, Victor ; Jiang, Guozhan ; Hill, David ; Williams, Craig ; Adamus, Grazyna ; Kwiecień, Michal ; Marek, Adam A ; Zawadiak, Jan ; Johnston, Brian ; Kowalczuk, Marek ; Teramoto, Naozumi (Editor). / Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production. In: Materials. 2016 ; Vol. 9, No. 5.
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abstract = "We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol {\%} of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.",
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Radecka, I, Irorere, V, Jiang, G, Hill, D, Williams, C, Adamus, G, Kwiecień, M, Marek, AA, Zawadiak, J, Johnston, B, Kowalczuk, M & Teramoto, N (ed.) 2016, 'Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production', Materials, vol. 9, no. 5. https://doi.org/10.3390/ma9050367

Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production. / Radecka, Iza; Irorere, Victor; Jiang, Guozhan; Hill, David; Williams, Craig; Adamus, Grazyna; Kwiecień, Michal; Marek, Adam A; Zawadiak, Jan; Johnston, Brian; Kowalczuk, Marek; Teramoto, Naozumi (Editor).

In: Materials, Vol. 9, No. 5, 05.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production

AU - Radecka, Iza

AU - Irorere, Victor

AU - Jiang, Guozhan

AU - Hill, David

AU - Williams, Craig

AU - Adamus, Grazyna

AU - Kwiecień, Michal

AU - Marek, Adam A

AU - Zawadiak, Jan

AU - Johnston, Brian

AU - Kowalczuk, Marek

A2 - Teramoto, Naozumi

PY - 2016/5

Y1 - 2016/5

N2 - We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.

AB - We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.

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DO - 10.3390/ma9050367

M3 - Article

VL - 9

JO - Materials

T2 - Materials

JF - Materials

SN - 1996-1944

IS - 5

ER -

Radecka I, Irorere V, Jiang G, Hill D, Williams C, Adamus G et al. Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production. Materials. 2016 May;9(5). https://doi.org/10.3390/ma9050367