Osteoblastic differentiation of periodontal ligament stem cells on non-stoichiometric calcium phosphate and titanium surfaces

L Winning, L Robinson, A. R. Boyd, I A El Karim, F T Lundy, BJ Meenan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1 +ve periodontal ligament stem cells (PDLSCs) to three well-characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi). The CaP-TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic-inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q-PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP-TiTi surfaces showed a significant late stage up-regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up-regulation of the Wnt signaling genes β-catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP-TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP-TiTi surface was also observed. These data suggest that the CaP-TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs.
LanguageEnglish
Pages1692-1702
JournalJournal of Biomedical Materials Research: Part A
Volume105
Issue number6
Early online date20 Feb 2017
DOIs
Publication statusPublished - Jun 2017

Fingerprint

Periodontal Ligament
Titanium
Stem Cells
Alkaline Phosphatase
Up-Regulation
Gene Expression
Osseointegration
Osteopontin
beta Catenin
Biocompatible Materials
Radio
Real-Time Polymerase Chain Reaction
Tooth
Calcium
calcium phosphate
Genes

Keywords

  • periodontal ligament stem cells
  • radio frequency magnetron sputtering
  • hydroxyapatite
  • osteoblastic differentiation
  • dental implants

Cite this

@article{4c8727b6b345478e81d125a2eea4b407,
title = "Osteoblastic differentiation of periodontal ligament stem cells on non-stoichiometric calcium phosphate and titanium surfaces",
abstract = "Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1 +ve periodontal ligament stem cells (PDLSCs) to three well-characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi). The CaP-TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic-inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q-PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP-TiTi surfaces showed a significant late stage up-regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up-regulation of the Wnt signaling genes β-catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP-TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP-TiTi surface was also observed. These data suggest that the CaP-TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs.",
keywords = "periodontal ligament stem cells, radio frequency magnetron sputtering, hydroxyapatite, osteoblastic differentiation, dental implants",
author = "L Winning and L Robinson and Boyd, {A. R.} and {El Karim}, {I A} and Lundy, {F T} and BJ Meenan",
note = "Compliant in UIR; evidence uploaded to 'Other files' Reference text: 1 Olivares-Navarrete R, Hyzy SL, Park JH, Dunn GR, Haithcock DA, Wasilewski CE, Boyan BD, Schwartz Z. Mediation of osteogenic differentiation of human mesenchymal stem cells on titanium surfaces by a Wnt-integrin feedback loop. Biomaterials 2011;32:6399–6411. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 50 2 Rausch-fan X, Qu Z, Wieland M, Matejka M, Schedle A. Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells (MG63) in response to titanium surfaces. Dent Mater 2008 ;24:102–110. Jan CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 71 3 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149–155. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 1144 4 Heo YY, Um S, Kim SK, Park JM, Seo BM. Responses of periodontal ligament stem cells on various titanium surfaces. Oral Dis 2011;17:320–327. Wiley Online Library | PubMed | Web of Science{\circledR} Times Cited: 10 5 Kim SY, Yoo JY, Ohe JY, Lee JW, Moon JH, Kwon YD, Heo JS. Differential expression of osteo-modulatory molecules in periodontal ligament stem cells in response to modified titanium surfaces. Biomed Res Int 2014;2014:452175. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 4 6 Li X, Liao D, Gong P, Dong Y, Sun G. Biological behavior of neur2ally differentiated periodontal ligament stem cells on different titanium implant surfaces. J Biomed Mater Res A 2014;102:2805–2812. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 2 7 Gao H, Li B, Zhao L, Jin Y. Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration. Int J Nanomed 2015 ;10:4009–4027. Web of Science{\circledR} Times Cited: 5 8 Albrektsson T, Johansson C. Osteoinduction, osteoconduction and osseointegration. Eur Spine J 2001;Suppl 2:S96–S101. 9 Olivares-Navarrete R, Hyzy SL, Hutton DL, Erdman CP, Wieland M, Boyan BD, Schwartz Z. Direct and indirect effects of microstructured titanium substrates on the induction of mesenchymal stem cell differentiation towards the osteoblast lineage. Biomaterials 2010;31:2728–2735. Apr CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 129 10 Sj{\"o}str{\"o}m T, Brydone AS, Meek RD, Dalby MJ, Su B, Mcnamara LE. Titanium nanofeaturing for enhanced bioactivity of implanted orthopedic and dental devices. Nanomedicine 2013;8:89–104. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 16 11 McCafferty MM, Burke GA, Meenan BJ. Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces. J Biomed Mater Res A 2014;102:3585–3597. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 4 12 Lobo SE, Glickman R, da Silva WN, Arinzeh TL, Kerkis I. Response of stem cells from different origins to biphasic calcium phosphate bioceramics. Cell Tissue Res 2015;361:477–495. Aug CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 2 13 Boyd AR, O'Kane C, O'Hare P, Burke GA, Meenan BJ. The influence of target stoichiometry on early cell adhesion of co-sputtered calcium-phosphate surfaces. J Mater Sci: Mater Med 2013;24:2845–2861. PubMed | Web of Science{\circledR} Times Cited: 3 14 Boyd AR, Meenan BJ, Leyland NS. Surface Characterization of the evolving nature of radio frequency (RF) magnetron sputter deposited calcium phosphate thin films after exposure to physiological solution. Surf Coat Technol 2006;200:6002–6013. CrossRef | CAS | Web of Science{\circledR} Times Cited: 49 15 O'Kane C, Duffy H, Meenan BJ, Boyd AR. The influence of target stoichiometry on the surface properties of sputter deposited calcium phosphate thin films. Surf Coat Technol 2008;203:121–128. CrossRef | CAS | Web of Science{\circledR} Times Cited: 12 16 Boyd AR, Rutledge L, Randolph LD, Mutreja I, Meenan BJ. The deposition of strontium-substituted hydroxyapatite coatings. J Mater Sci: Mater Med 2015;26:65. PubMed | Web of Science{\circledR} Times Cited: 2 17 Boyd AR, Duffy H, McCann R, Meenan BJ. Sputter deposition of calcium phosphate/titanium dioxide hybrid thin films. Mater Sci Eng C 2008;28:228–236. CrossRef | CAS | Web of Science{\circledR} Times Cited: 25 18 Boyd AR, Burke GA, Duffy H, Cairns ML, O'Hare P, Meenan BJ. Characterization of calcium phosphate/titanium dioxide hybrid coatings. J Mater Sci: Mater Med 2008;19:485–498. PubMed | Web of Science{\circledR} Times Cited: 11 19 Zhao L, Liu L, Wu Z, Zhang Y, Chu PK. Effects of micropitted/nanotubular titania topographies on bone mesenchymal stem cell osteogenic differentiation. Biomaterials 2012 ;33:2629–2641. Mar CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 111 20 McCafferty MM, Burke GA, Meenan BJ. Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces. J Biomed Mater Res A 2014;102:3585–3597. Oct Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 4 21 Wopenka B, Pasteris JD. A mineralogical perspective on the apatite in bone. Mater Sci Eng C 2005;25:131–143. CrossRef | CAS | Web of Science{\circledR} Times Cited: 288 22 Gibson IR, Bonfield W. Novel synthesis and characterization of an AB-type carbonate-substituted hydroxyapatite. J Biomed Mater Res 2002;59:697–708. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 205 23 Vallet-Reg{\'i} M, Gonz{\'a}lez-Calbet JM. Calcium phosphates as substitution of bone tissues. Prog Solid State Chem 2004;32:1–31. CrossRef | CAS | Web of Science{\circledR} Times Cited: 475 24 Kim H, Camata RP, Chowdhury S, Vohra YK. In vitro dissolution and mechanical behavior of c-axis preferentially oriented hydroxyapatite thin films fabricated by pulsed laser deposition. Acta Biomater 2010;6:3234–3241. Aug CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 34 25 Geesink RG, de Groot K, Klein CP. Bonding of bone to apatite-coated implants. J Bone Joint Surg Br 1988;70:17–22. PubMed | Web of Science{\circledR} Times Cited: 371 26 Guo Y, Yao Y, Ning C, Guo Y, Chu L. Fabrication of mesoporous carbonated hydroxyapatite microspheres by hydrothermal method. Mater Lett 2011;65:2205–2208. CrossRef | CAS | Web of Science{\circledR} Times Cited: 25 27 Magne D, Pilet P, Weiss P, Daculsi G. Fourier transform infrared microspectroscopic investigation of the maturation of nonstoichiometric apatites in mineralized tissues: A horse dentin study. Bone 2001;29:547–552. Dec CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 21 28 Wu J, Hayakawa S, Tsuru K, Osaka A. Low-temperature preparation of anatase and rutile layers on titanium substrates and their ability to induce in vitro apatite deposition. J Am Ceram Soc 2004;87:1635–1642. Wiley Online Library | CAS | Web of Science{\circledR} Times Cited: 113 29 Lovmand J, Justesen J, Foss M, Lauridsen RH, Lovmand M, Modin C, Besenbacher F, Pedersen FS, Duch M. The use of combinatorial topographical libraries for the screening of enhanced osteogenic expression and mineralization. Biomaterials 2009 ;30:2015–2022. Apr CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 61 30 Kulterer B, Friedl G, Jandrositz A, Sanchez-Cabo F, Prokesch A, Paar C, Scheideler M, Windhager R, Preisegger KH, Trajanoski Z. Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation. BMC Genomics 2007;8:70. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 160 31 Aubin JE. Regulation of osteoblast formation and function. Rev Endocr Metab Disord 2001;2:81–94. CrossRef | PubMed | CAS 32 Dorozhkin SV. Surface reactions of apatite dissolution. J Colloid Interface Sci 1997;191:489–497. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 48 33 Dorozhkin SV. A review on the dissolution models of calcium apatites. Prog Cryst Growth Charact Mater 2002;44:45–61. CrossRef | CAS | Web of Science{\circledR} Times Cited: 100 34 Shui C, Spelsberg TC, Riggs BL, Khosla S. Changes in Runx2/Cbfa1 expression and activity during osteoblastic differentiation of human bone marrow stromal cells. J Bone Miner Res 2003;18:213–221. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 144 35 Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res 2010;339:189–195. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 256 36 Oliveira NC, Moura CC, Zanetta-Barbosa D, Mendonca DB, Cooper L, Mendonca G, Dechichi P. Effects of titanium surface anodization with CaP incorporation on human osteoblastic response. Mater Sci Eng C Mater Biol Appl 2013;33:1958–1962. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 9 37 Mendonca G, Mendonca DB, Simoes LG, Araujo AL, Leite ER, Duarte WR, Aragao FJ, Cooper LF. The effects of implant surface nanoscale features on osteoblast-specific gene expression. Biomaterials 2009;30:4053–4062. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 121 38 Mendonca G, Mendonca DB, Aragao FJ, Cooper LF. The combination of micron and nanotopography by H(2)SO(4)/H(2)O(2) treatment and its effects on osteoblast-specific gene expression of hMSCs. J Biomed Mater Res A 2010;94:169–179. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 51 39 Jikko A, Harris SE, Chen D, Mendrick DL, Damsky CH. Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 1999;14:1075–1083. Wiley Online Library | PubMed | CAS | Web of Science{\circledR} Times Cited: 149 40 Bodine PV, Komm BS. Wnt signaling and osteoblastogenesis. Rev Endocr Metab Disord 2006;7:33–39. CrossRef | PubMed | CAS | Web of Science{\circledR} Times Cited: 165",
year = "2017",
month = "6",
doi = "10.1002/jbm.a.36044",
language = "English",
volume = "105",
pages = "1692--1702",
journal = "Journal of Biomedical Materials Research: Part A",
issn = "1549-3296",
number = "6",

}

Osteoblastic differentiation of periodontal ligament stem cells on non-stoichiometric calcium phosphate and titanium surfaces. / Winning, L; Robinson, L; Boyd, A. R.; El Karim, I A; Lundy, F T; Meenan, BJ.

In: Journal of Biomedical Materials Research: Part A, Vol. 105, No. 6, 06.2017, p. 1692-1702.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Osteoblastic differentiation of periodontal ligament stem cells on non-stoichiometric calcium phosphate and titanium surfaces

AU - Winning, L

AU - Robinson, L

AU - Boyd, A. R.

AU - El Karim, I A

AU - Lundy, F T

AU - Meenan, BJ

N1 - Compliant in UIR; evidence uploaded to 'Other files' Reference text: 1 Olivares-Navarrete R, Hyzy SL, Park JH, Dunn GR, Haithcock DA, Wasilewski CE, Boyan BD, Schwartz Z. Mediation of osteogenic differentiation of human mesenchymal stem cells on titanium surfaces by a Wnt-integrin feedback loop. Biomaterials 2011;32:6399–6411. CrossRef | PubMed | CAS | Web of Science® Times Cited: 50 2 Rausch-fan X, Qu Z, Wieland M, Matejka M, Schedle A. Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells (MG63) in response to titanium surfaces. Dent Mater 2008 ;24:102–110. Jan CrossRef | PubMed | CAS | Web of Science® Times Cited: 71 3 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149–155. CrossRef | PubMed | CAS | Web of Science® Times Cited: 1144 4 Heo YY, Um S, Kim SK, Park JM, Seo BM. Responses of periodontal ligament stem cells on various titanium surfaces. Oral Dis 2011;17:320–327. Wiley Online Library | PubMed | Web of Science® Times Cited: 10 5 Kim SY, Yoo JY, Ohe JY, Lee JW, Moon JH, Kwon YD, Heo JS. Differential expression of osteo-modulatory molecules in periodontal ligament stem cells in response to modified titanium surfaces. Biomed Res Int 2014;2014:452175. CrossRef | PubMed | CAS | Web of Science® Times Cited: 4 6 Li X, Liao D, Gong P, Dong Y, Sun G. Biological behavior of neur2ally differentiated periodontal ligament stem cells on different titanium implant surfaces. J Biomed Mater Res A 2014;102:2805–2812. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 2 7 Gao H, Li B, Zhao L, Jin Y. Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration. Int J Nanomed 2015 ;10:4009–4027. Web of Science® Times Cited: 5 8 Albrektsson T, Johansson C. Osteoinduction, osteoconduction and osseointegration. Eur Spine J 2001;Suppl 2:S96–S101. 9 Olivares-Navarrete R, Hyzy SL, Hutton DL, Erdman CP, Wieland M, Boyan BD, Schwartz Z. Direct and indirect effects of microstructured titanium substrates on the induction of mesenchymal stem cell differentiation towards the osteoblast lineage. Biomaterials 2010;31:2728–2735. Apr CrossRef | PubMed | CAS | Web of Science® Times Cited: 129 10 Sjöström T, Brydone AS, Meek RD, Dalby MJ, Su B, Mcnamara LE. Titanium nanofeaturing for enhanced bioactivity of implanted orthopedic and dental devices. Nanomedicine 2013;8:89–104. CrossRef | PubMed | CAS | Web of Science® Times Cited: 16 11 McCafferty MM, Burke GA, Meenan BJ. Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces. J Biomed Mater Res A 2014;102:3585–3597. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 4 12 Lobo SE, Glickman R, da Silva WN, Arinzeh TL, Kerkis I. Response of stem cells from different origins to biphasic calcium phosphate bioceramics. Cell Tissue Res 2015;361:477–495. Aug CrossRef | PubMed | CAS | Web of Science® Times Cited: 2 13 Boyd AR, O'Kane C, O'Hare P, Burke GA, Meenan BJ. The influence of target stoichiometry on early cell adhesion of co-sputtered calcium-phosphate surfaces. J Mater Sci: Mater Med 2013;24:2845–2861. PubMed | Web of Science® Times Cited: 3 14 Boyd AR, Meenan BJ, Leyland NS. Surface Characterization of the evolving nature of radio frequency (RF) magnetron sputter deposited calcium phosphate thin films after exposure to physiological solution. Surf Coat Technol 2006;200:6002–6013. CrossRef | CAS | Web of Science® Times Cited: 49 15 O'Kane C, Duffy H, Meenan BJ, Boyd AR. The influence of target stoichiometry on the surface properties of sputter deposited calcium phosphate thin films. Surf Coat Technol 2008;203:121–128. CrossRef | CAS | Web of Science® Times Cited: 12 16 Boyd AR, Rutledge L, Randolph LD, Mutreja I, Meenan BJ. The deposition of strontium-substituted hydroxyapatite coatings. J Mater Sci: Mater Med 2015;26:65. PubMed | Web of Science® Times Cited: 2 17 Boyd AR, Duffy H, McCann R, Meenan BJ. Sputter deposition of calcium phosphate/titanium dioxide hybrid thin films. Mater Sci Eng C 2008;28:228–236. CrossRef | CAS | Web of Science® Times Cited: 25 18 Boyd AR, Burke GA, Duffy H, Cairns ML, O'Hare P, Meenan BJ. Characterization of calcium phosphate/titanium dioxide hybrid coatings. J Mater Sci: Mater Med 2008;19:485–498. PubMed | Web of Science® Times Cited: 11 19 Zhao L, Liu L, Wu Z, Zhang Y, Chu PK. Effects of micropitted/nanotubular titania topographies on bone mesenchymal stem cell osteogenic differentiation. Biomaterials 2012 ;33:2629–2641. Mar CrossRef | PubMed | CAS | Web of Science® Times Cited: 111 20 McCafferty MM, Burke GA, Meenan BJ. Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces. J Biomed Mater Res A 2014;102:3585–3597. Oct Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 4 21 Wopenka B, Pasteris JD. A mineralogical perspective on the apatite in bone. Mater Sci Eng C 2005;25:131–143. CrossRef | CAS | Web of Science® Times Cited: 288 22 Gibson IR, Bonfield W. Novel synthesis and characterization of an AB-type carbonate-substituted hydroxyapatite. J Biomed Mater Res 2002;59:697–708. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 205 23 Vallet-Regí M, González-Calbet JM. Calcium phosphates as substitution of bone tissues. Prog Solid State Chem 2004;32:1–31. CrossRef | CAS | Web of Science® Times Cited: 475 24 Kim H, Camata RP, Chowdhury S, Vohra YK. In vitro dissolution and mechanical behavior of c-axis preferentially oriented hydroxyapatite thin films fabricated by pulsed laser deposition. Acta Biomater 2010;6:3234–3241. Aug CrossRef | PubMed | CAS | Web of Science® Times Cited: 34 25 Geesink RG, de Groot K, Klein CP. Bonding of bone to apatite-coated implants. J Bone Joint Surg Br 1988;70:17–22. PubMed | Web of Science® Times Cited: 371 26 Guo Y, Yao Y, Ning C, Guo Y, Chu L. Fabrication of mesoporous carbonated hydroxyapatite microspheres by hydrothermal method. Mater Lett 2011;65:2205–2208. CrossRef | CAS | Web of Science® Times Cited: 25 27 Magne D, Pilet P, Weiss P, Daculsi G. Fourier transform infrared microspectroscopic investigation of the maturation of nonstoichiometric apatites in mineralized tissues: A horse dentin study. Bone 2001;29:547–552. Dec CrossRef | PubMed | CAS | Web of Science® Times Cited: 21 28 Wu J, Hayakawa S, Tsuru K, Osaka A. Low-temperature preparation of anatase and rutile layers on titanium substrates and their ability to induce in vitro apatite deposition. J Am Ceram Soc 2004;87:1635–1642. Wiley Online Library | CAS | Web of Science® Times Cited: 113 29 Lovmand J, Justesen J, Foss M, Lauridsen RH, Lovmand M, Modin C, Besenbacher F, Pedersen FS, Duch M. The use of combinatorial topographical libraries for the screening of enhanced osteogenic expression and mineralization. Biomaterials 2009 ;30:2015–2022. Apr CrossRef | PubMed | CAS | Web of Science® Times Cited: 61 30 Kulterer B, Friedl G, Jandrositz A, Sanchez-Cabo F, Prokesch A, Paar C, Scheideler M, Windhager R, Preisegger KH, Trajanoski Z. Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation. BMC Genomics 2007;8:70. CrossRef | PubMed | CAS | Web of Science® Times Cited: 160 31 Aubin JE. Regulation of osteoblast formation and function. Rev Endocr Metab Disord 2001;2:81–94. CrossRef | PubMed | CAS 32 Dorozhkin SV. Surface reactions of apatite dissolution. J Colloid Interface Sci 1997;191:489–497. CrossRef | PubMed | CAS | Web of Science® Times Cited: 48 33 Dorozhkin SV. A review on the dissolution models of calcium apatites. Prog Cryst Growth Charact Mater 2002;44:45–61. CrossRef | CAS | Web of Science® Times Cited: 100 34 Shui C, Spelsberg TC, Riggs BL, Khosla S. Changes in Runx2/Cbfa1 expression and activity during osteoblastic differentiation of human bone marrow stromal cells. J Bone Miner Res 2003;18:213–221. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 144 35 Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res 2010;339:189–195. CrossRef | PubMed | CAS | Web of Science® Times Cited: 256 36 Oliveira NC, Moura CC, Zanetta-Barbosa D, Mendonca DB, Cooper L, Mendonca G, Dechichi P. Effects of titanium surface anodization with CaP incorporation on human osteoblastic response. Mater Sci Eng C Mater Biol Appl 2013;33:1958–1962. CrossRef | PubMed | CAS | Web of Science® Times Cited: 9 37 Mendonca G, Mendonca DB, Simoes LG, Araujo AL, Leite ER, Duarte WR, Aragao FJ, Cooper LF. The effects of implant surface nanoscale features on osteoblast-specific gene expression. Biomaterials 2009;30:4053–4062. CrossRef | PubMed | CAS | Web of Science® Times Cited: 121 38 Mendonca G, Mendonca DB, Aragao FJ, Cooper LF. The combination of micron and nanotopography by H(2)SO(4)/H(2)O(2) treatment and its effects on osteoblast-specific gene expression of hMSCs. J Biomed Mater Res A 2010;94:169–179. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 51 39 Jikko A, Harris SE, Chen D, Mendrick DL, Damsky CH. Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 1999;14:1075–1083. Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 149 40 Bodine PV, Komm BS. Wnt signaling and osteoblastogenesis. Rev Endocr Metab Disord 2006;7:33–39. CrossRef | PubMed | CAS | Web of Science® Times Cited: 165

PY - 2017/6

Y1 - 2017/6

N2 - Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1 +ve periodontal ligament stem cells (PDLSCs) to three well-characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi). The CaP-TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic-inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q-PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP-TiTi surfaces showed a significant late stage up-regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up-regulation of the Wnt signaling genes β-catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP-TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP-TiTi surface was also observed. These data suggest that the CaP-TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs.

AB - Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1 +ve periodontal ligament stem cells (PDLSCs) to three well-characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi). The CaP-TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic-inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q-PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP-TiTi surfaces showed a significant late stage up-regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up-regulation of the Wnt signaling genes β-catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP-TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP-TiTi surface was also observed. These data suggest that the CaP-TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs.

KW - periodontal ligament stem cells

KW - radio frequency magnetron sputtering

KW - hydroxyapatite

KW - osteoblastic differentiation

KW - dental implants

UR - http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36044/full

UR - https://pure.ulster.ac.uk/en/publications/osteoblastic-differentiation-of-periodontal-ligament-stem-cells-o-3

U2 - 10.1002/jbm.a.36044

DO - 10.1002/jbm.a.36044

M3 - Article

VL - 105

SP - 1692

EP - 1702

JO - Journal of Biomedical Materials Research: Part A

T2 - Journal of Biomedical Materials Research: Part A

JF - Journal of Biomedical Materials Research: Part A

SN - 1549-3296

IS - 6

ER -