TY - JOUR
T1 - Organophosphonate metabolism by a moderately halophilic bacterial isolate
AU - Hayes, VEA
AU - Ternan, NG
AU - McMullan, Geoffrey
PY - 2000/5
Y1 - 2000/5
N2 - A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethyiphos-phonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
AB - A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethyiphos-phonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
U2 - 10.1111/j.1574-6968.2000.tb09099.x
DO - 10.1111/j.1574-6968.2000.tb09099.x
M3 - Article
SN - 1574-6968
VL - 186
SP - 171
EP - 175
JO - Fems Microbiology Letters
JF - Fems Microbiology Letters
IS - 2
ER -