Organophosphonate metabolism by a moderately halophilic bacterial isolate

VEA Hayes, NG Ternan, Geoffrey McMullan

Research output: Contribution to journalArticle

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Abstract

A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethyiphos-phonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
LanguageEnglish
Pages171-175
JournalFems Microbiology Letters
Volume186
Issue number2
DOIs
Publication statusPublished - May 2000

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phosphonates
Pseudomonas beijerinckii
Chromohalobacter marismortui
metabolism
transaminases
hydrolases
roads
ribosomal RNA
cells
salts
phosphorus
extracts
assays
soil

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Hayes, VEA ; Ternan, NG ; McMullan, Geoffrey. / Organophosphonate metabolism by a moderately halophilic bacterial isolate. In: Fems Microbiology Letters. 2000 ; Vol. 186, No. 2. pp. 171-175.
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Organophosphonate metabolism by a moderately halophilic bacterial isolate. / Hayes, VEA; Ternan, NG; McMullan, Geoffrey.

In: Fems Microbiology Letters, Vol. 186, No. 2, 05.2000, p. 171-175.

Research output: Contribution to journalArticle

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