Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

Mohammadhossein Ahmadi  Borhanabadi; Mohammad amin Raeist Estabragh; Gholamreza Dehghannoudeh; Ibrahim M Banat; Mandana Ohadi; Mohammad Hassan Moshafi

doi:10.5812/jjnpp-134325

Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

Mohammadhossein Ahmadi Borhanabadi
, Mohammad amin Raeist Estabragh
, Gholamreza Dehghannoudeh
, Ibrahim M Banat
, Mandana Ohadi
, Mohammad Hassan Moshafi

Kerman University of Medical Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

88 Downloads (Pure)

Abstract

Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties.
Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel.
Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s
morphology.
Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design.
Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.

Original language	English
Article number	134325
Pages (from-to)	1-8
Number of pages	8
Journal	Jundishapur Journal of Natural Pharmaceutical Products
Volume	18
Issue number	2
Early online date	13 May 2023
DOIs	https://doi.org/10.5812/jjnpp-134325
Publication status	Published online - 13 May 2023

Bibliographical note

Funding Information:
This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University.

Publisher Copyright:
© 2023, Author(s).

Funding

Funding Information: This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University. Publisher Copyright: © 2023, Author(s).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acinetobacter junii B6
Bioencapsulation
Biosurfactant
Calcium Alginate
Optimization

Access to Document

10.5812/jjnpp-134325

Calcium Alginate Hdrogel Bioencapsulation paper ProofsFinal published version, 1.41 MBLicence: CC BY-NC

Cite this

Borhanabadi, M. A., Estabragh, M. A. R., Dehghannoudeh, G., Banat, I. M., Ohadi, M., & Moshafi, M. H. (2023). Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer. Jundishapur Journal of Natural Pharmaceutical Products, 18(2), 1-8. Article 134325. Advance online publication. https://doi.org/10.5812/jjnpp-134325

@article{80dfce432183400e8c5faccedf8a09b4,

title = "Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer",

abstract = "Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties. Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel. Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead{\textquoteright}s morphology. Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1\% calcium chloride, (1\%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design. Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.",

keywords = "Acinetobacter junii B6, Bioencapsulation, Biosurfactant, Calcium Alginate, Optimization",

author = "Borhanabadi, \{Mohammadhossein Ahmadi\} and Estabragh, \{Mohammad amin Raeist\} and Gholamreza Dehghannoudeh and Banat, \{Ibrahim M\} and Mandana Ohadi and Moshafi, \{Mohammad Hassan\}",

note = "Funding Information: This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University. Publisher Copyright: {\textcopyright} 2023, Author(s).",

year = "2023",

month = may,

day = "13",

doi = "10.5812/jjnpp-134325",

language = "English",

volume = "18",

pages = "1--8",

journal = "Jundishapur Journal of Natural Pharmaceutical Products",

issn = "2228-7876",

publisher = "Brieflands",

number = "2",

}

Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer. / Borhanabadi, Mohammadhossein Ahmadi ; Estabragh, Mohammad amin Raeist; Dehghannoudeh, Gholamreza et al.
In: Jundishapur Journal of Natural Pharmaceutical Products, Vol. 18, No. 2, 134325, 13.05.2023, p. 1-8.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

AU - Borhanabadi, Mohammadhossein Ahmadi

AU - Estabragh, Mohammad amin Raeist

AU - Dehghannoudeh, Gholamreza

AU - Banat, Ibrahim M

AU - Ohadi, Mandana

AU - Moshafi, Mohammad Hassan

N1 - Funding Information: This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University. Publisher Copyright: © 2023, Author(s).

PY - 2023/5/13

Y1 - 2023/5/13

N2 - Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties. Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel. Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s morphology. Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design. Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.

AB - Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties. Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel. Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s morphology. Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design. Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.

KW - Acinetobacter junii B6

KW - Bioencapsulation

KW - Biosurfactant

KW - Calcium Alginate

KW - Optimization

UR - https://www.scopus.com/pages/publications/85162741128

U2 - 10.5812/jjnpp-134325

DO - 10.5812/jjnpp-134325

M3 - Article

SN - 2228-7876

VL - 18

SP - 1

EP - 8

JO - Jundishapur Journal of Natural Pharmaceutical Products

JF - Jundishapur Journal of Natural Pharmaceutical Products

IS - 2

M1 - 134325

ER -

Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this