Abstract
Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties.
Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel.
Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s
morphology.
Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design.
Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.
Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel.
Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s
morphology.
Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design.
Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.
| Original language | English |
|---|---|
| Article number | 134325 |
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Jundishapur Journal of Natural Pharmaceutical Products |
| Volume | 18 |
| Issue number | 2 |
| Early online date | 13 May 2023 |
| DOIs | |
| Publication status | Published online - 13 May 2023 |
Bibliographical note
Funding Information:This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University.
Publisher Copyright:
© 2023, Author(s).
Keywords
- Acinetobacter junii B6
- Bioencapsulation
- Biosurfactant
- Calcium Alginate
- Optimization
