Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

Mohammadhossein Ahmadi Borhanabadi, Mohammad amin Raeist Estabragh, Gholamreza Dehghannoudeh, Ibrahim M Banat, Mandana Ohadi, Mohammad Hassan Moshafi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
10 Downloads (Pure)


Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties.
Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel.
Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead’s
Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hard-ening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design.
Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants.
Original languageEnglish
Article number134325
Pages (from-to)1-8
Number of pages8
JournalJundishapur Journal of Natural Pharmaceutical Products
Issue number2
Early online date13 May 2023
Publication statusPublished online - 13 May 2023

Bibliographical note

Funding Information:
This article is the result of a research project approved by the Student Research Committee of Kerman University of Medical Sciences No. 402000029, which was carried out with the financial support of the Vice-Chancellor for Research and Technology of the University.

Publisher Copyright:
© 2023, Author(s).


  • Acinetobacter junii B6
  • Bioencapsulation
  • Biosurfactant
  • Calcium Alginate
  • Optimization


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