Optimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: Potential applications for gene therapy of cancer

Ying Suet Li, Emma Davidson, Cherith N. Reid, Anthony McHale

Research output: Contribution to journalArticle

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Abstract

Therapeutic approaches using gene-based medicines promise alternatives or adjuncts to conventional cancer treatment. Because of its non-invasive nature, ultrasound, as a membrane-permeabilising stimulus has the potential to be highly competitive with viral gene delivery and existing non-viral alternatives. In optimising ultrasound-mediated, microbubble-assisted (MB101) gene tranfection in vitro. we demonstrate efficiencies of up to 18% using ultrasound at 1 MHz at a duty cycle of 25% at intensities ranging from 1 to 4 W cm(-2). Using ultrasound-mediated transfection together with an episomal plasmid-based gene expression system, we demonstrate prolonged functional gene expression of luciferase in mouse hind leg muscle and in tumours in vivo. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.
LanguageEnglish
Pages62-69
JournalCancer Letters
Volume273
Issue number1
DOIs
Publication statusPublished - Jan 2009

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Transgenes
Genetic Therapy
Gene Expression
Microbubbles
Viral Genes
Complementary Therapies
Luciferases
Crowns
Genes
Transfection
Neoplasms
Leg
Plasmids
Muscles
Membranes
Therapeutics
In Vitro Techniques

Cite this

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abstract = "Therapeutic approaches using gene-based medicines promise alternatives or adjuncts to conventional cancer treatment. Because of its non-invasive nature, ultrasound, as a membrane-permeabilising stimulus has the potential to be highly competitive with viral gene delivery and existing non-viral alternatives. In optimising ultrasound-mediated, microbubble-assisted (MB101) gene tranfection in vitro. we demonstrate efficiencies of up to 18{\%} using ultrasound at 1 MHz at a duty cycle of 25{\%} at intensities ranging from 1 to 4 W cm(-2). Using ultrasound-mediated transfection together with an episomal plasmid-based gene expression system, we demonstrate prolonged functional gene expression of luciferase in mouse hind leg muscle and in tumours in vivo. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.",
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Optimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: Potential applications for gene therapy of cancer. / Li, Ying Suet; Davidson, Emma; Reid, Cherith N.; McHale, Anthony.

In: Cancer Letters, Vol. 273, No. 1, 01.2009, p. 62-69.

Research output: Contribution to journalArticle

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