One-step hydrolysis ethanol fermentation of cellobiose and pinewood-cellulose by cell factories of non-GMO saccharomyces cerevisiae using kissiris and γ-alumina as support

This project aimed to compare the efficiencies of cell factories designed using non-engineered cells of Saccharomyces cerevisiae to conduct a one-step process of simultaneous hydrolysis and fermentation of cellobiose to ethanol. The investigation involved the preparation of CFs of S. cerevisiae cells covered by starch gel and mineral kissiris or γ-alumina with a cellulolytic agent Trichoderma reesei, or a commercial cellulase, or our lab-preparation of cellulase. Analysis showed that high ethanol yield (up to 82%) was achieved in a 72-hours one-step process using CFs S. cerevisiae/SG-commercial cellulases, and S. cerevisiae-alu kis/SG-commercial cellulases, indicating the efficiency of CFs. Thereafter, the CF with the best performance was used for cellulose to bioethanol in one-step resulting in 61.7% cellulose bioconversion. In our studies, the ethanol yield and substrate bioconversion were found competitive, with another research reported using engineered S. cerevisiae and co-immobilization. We used non-engineered yeast cells, cheaper material kissiris, and our lab-preparation of cellulases.