Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function

John van Tuyn, Farah Jaber-Hijazi, Douglas MacKenzie, John J. Cole, Elizabeth Mann, Jeff S. Pawlikowski, Taranjit Singh Rai, David M. Nelson, Tony McBryan, Andre Ivanov, Karen Blyth, Hong Wu, Simon Milling, Peter D. Adams

Research output: Contribution to journalArticle

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Abstract

On acquisition of an oncogenic mutation, primary human and mouse cells can enter oncogene-induced senescence (OIS). OIS is characterized by a stable proliferation arrest and secretion of proinflammatory cytokines and chemokines, the senescence-associated secretory phenotype. Proliferation arrest and the senescence-associated secretory phenotype collaborate to enact tumor suppression, the former by blocking cell proliferation and the latter by recruiting immune cells to clear damaged cells. However, the interactions of OIS cells with the immune system are still poorly defined. Here, we show that engagement of OIS in primary human melanocytes, specifically by melanoma driver mutations NRASQ61K and BRAFV600E, causes expression of the major histocompatibility class II antigen presentation apparatus, via secreted IL-1ß signaling and expression of CIITA, a master regulator of major histocompatibility class II gene transcription. In vitro, OIS melanocytes activate T-cell proliferation. In vivo, nonproliferating oncogene-expressing melanocytes localize to skin-draining lymph nodes, where they induce T-cell proliferation and an antigen presentation gene expression signature. In patients, expression of major histocompatibility class II in melanoma is linked to favorable disease outcome. We propose that OIS in melanocytes is accompanied by an antigen presentation phenotype, likely to promote tumor suppression via activation of the adaptive immune system.

LanguageEnglish
Pages2197-2207
Number of pages11
JournalJournal of Investigative Dermatology
Volume137
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

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Melanocytes
Cell proliferation
Oncogenes
Melanoma
Up-Regulation
T-cells
Immune system
Tumors
Histocompatibility
Antigen Presentation
Antigens
Histocompatibility Antigens Class II
Transcription
Chemokines
Gene expression
Cell Proliferation
Phenotype
Skin
Genes
Chemical activation

Cite this

van Tuyn, J., Jaber-Hijazi, F., MacKenzie, D., Cole, J. J., Mann, E., Pawlikowski, J. S., ... Adams, P. D. (2017). Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function. Journal of Investigative Dermatology, 137(10), 2197-2207. https://doi.org/10.1016/j.jid.2017.05.030
van Tuyn, John ; Jaber-Hijazi, Farah ; MacKenzie, Douglas ; Cole, John J. ; Mann, Elizabeth ; Pawlikowski, Jeff S. ; Rai, Taranjit Singh ; Nelson, David M. ; McBryan, Tony ; Ivanov, Andre ; Blyth, Karen ; Wu, Hong ; Milling, Simon ; Adams, Peter D. / Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function. In: Journal of Investigative Dermatology. 2017 ; Vol. 137, No. 10. pp. 2197-2207.
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van Tuyn, J, Jaber-Hijazi, F, MacKenzie, D, Cole, JJ, Mann, E, Pawlikowski, JS, Rai, TS, Nelson, DM, McBryan, T, Ivanov, A, Blyth, K, Wu, H, Milling, S & Adams, PD 2017, 'Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function', Journal of Investigative Dermatology, vol. 137, no. 10, pp. 2197-2207. https://doi.org/10.1016/j.jid.2017.05.030

Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function. / van Tuyn, John; Jaber-Hijazi, Farah; MacKenzie, Douglas; Cole, John J.; Mann, Elizabeth; Pawlikowski, Jeff S.; Rai, Taranjit Singh; Nelson, David M.; McBryan, Tony; Ivanov, Andre; Blyth, Karen; Wu, Hong; Milling, Simon; Adams, Peter D.

In: Journal of Investigative Dermatology, Vol. 137, No. 10, 01.10.2017, p. 2197-2207.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oncogene-Expressing Senescent Melanocytes Up-Regulate MHC Class II, a Candidate Melanoma Suppressor Function

AU - van Tuyn, John

AU - Jaber-Hijazi, Farah

AU - MacKenzie, Douglas

AU - Cole, John J.

AU - Mann, Elizabeth

AU - Pawlikowski, Jeff S.

AU - Rai, Taranjit Singh

AU - Nelson, David M.

AU - McBryan, Tony

AU - Ivanov, Andre

AU - Blyth, Karen

AU - Wu, Hong

AU - Milling, Simon

AU - Adams, Peter D.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - On acquisition of an oncogenic mutation, primary human and mouse cells can enter oncogene-induced senescence (OIS). OIS is characterized by a stable proliferation arrest and secretion of proinflammatory cytokines and chemokines, the senescence-associated secretory phenotype. Proliferation arrest and the senescence-associated secretory phenotype collaborate to enact tumor suppression, the former by blocking cell proliferation and the latter by recruiting immune cells to clear damaged cells. However, the interactions of OIS cells with the immune system are still poorly defined. Here, we show that engagement of OIS in primary human melanocytes, specifically by melanoma driver mutations NRASQ61K and BRAFV600E, causes expression of the major histocompatibility class II antigen presentation apparatus, via secreted IL-1ß signaling and expression of CIITA, a master regulator of major histocompatibility class II gene transcription. In vitro, OIS melanocytes activate T-cell proliferation. In vivo, nonproliferating oncogene-expressing melanocytes localize to skin-draining lymph nodes, where they induce T-cell proliferation and an antigen presentation gene expression signature. In patients, expression of major histocompatibility class II in melanoma is linked to favorable disease outcome. We propose that OIS in melanocytes is accompanied by an antigen presentation phenotype, likely to promote tumor suppression via activation of the adaptive immune system.

AB - On acquisition of an oncogenic mutation, primary human and mouse cells can enter oncogene-induced senescence (OIS). OIS is characterized by a stable proliferation arrest and secretion of proinflammatory cytokines and chemokines, the senescence-associated secretory phenotype. Proliferation arrest and the senescence-associated secretory phenotype collaborate to enact tumor suppression, the former by blocking cell proliferation and the latter by recruiting immune cells to clear damaged cells. However, the interactions of OIS cells with the immune system are still poorly defined. Here, we show that engagement of OIS in primary human melanocytes, specifically by melanoma driver mutations NRASQ61K and BRAFV600E, causes expression of the major histocompatibility class II antigen presentation apparatus, via secreted IL-1ß signaling and expression of CIITA, a master regulator of major histocompatibility class II gene transcription. In vitro, OIS melanocytes activate T-cell proliferation. In vivo, nonproliferating oncogene-expressing melanocytes localize to skin-draining lymph nodes, where they induce T-cell proliferation and an antigen presentation gene expression signature. In patients, expression of major histocompatibility class II in melanoma is linked to favorable disease outcome. We propose that OIS in melanocytes is accompanied by an antigen presentation phenotype, likely to promote tumor suppression via activation of the adaptive immune system.

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