Occurrence of crude oil degrading bacteria in gasoline and diesel station soils

KSM Rahman, T Rahman, P Lakshmanaperumalsamy, Ibrahim Banat

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Microbial enumeration and identification were carried out on several oil contaminated soil samples collected from gasoline and diesel stations. Bacteria were the most dominant microbiota and were therefore classified to generic level. Eleven main genera were detected and Corynebacterium was the predominant genus in all the samples. Biochemical characterisation and substrate utilisation showed high percentage of lipolytic ability combined with high inorganic nitrogen utilisers. The ability of these cultures to degrade crude oil was tested individually and in mixed bacterial consortium at different temperatures and pH values. Maximum crude oil biodegradation of 78% was achieved using a bacterial consortium containing five cultures (Micrococcus sp. GS2-22, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Bacillus sp. DS6-86 and Pseudomonas sp. DS10-129) with 1% crude oil at 30 degreesC and pH 7.5. Such a consortium may be useful for bioaugmentation of oil contaminated environments.
LanguageEnglish
Pages284-291
JournalJOURNAL OF BASIC MICROBIOLOGY
Volume42
Issue number4
Publication statusPublished - 2002

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diesel
crude oil
bacterium
soil
oil
inorganic nitrogen
biodegradation
substrate
station
temperature
contaminated soil
pH-value

Cite this

Rahman, KSM ; Rahman, T ; Lakshmanaperumalsamy, P ; Banat, Ibrahim. / Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. In: JOURNAL OF BASIC MICROBIOLOGY. 2002 ; Vol. 42, No. 4. pp. 284-291.
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Rahman, KSM, Rahman, T, Lakshmanaperumalsamy, P & Banat, I 2002, 'Occurrence of crude oil degrading bacteria in gasoline and diesel station soils', JOURNAL OF BASIC MICROBIOLOGY, vol. 42, no. 4, pp. 284-291.

Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. / Rahman, KSM; Rahman, T; Lakshmanaperumalsamy, P; Banat, Ibrahim.

In: JOURNAL OF BASIC MICROBIOLOGY, Vol. 42, No. 4, 2002, p. 284-291.

Research output: Contribution to journalArticle

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T1 - Occurrence of crude oil degrading bacteria in gasoline and diesel station soils

AU - Rahman, KSM

AU - Rahman, T

AU - Lakshmanaperumalsamy, P

AU - Banat, Ibrahim

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AB - Microbial enumeration and identification were carried out on several oil contaminated soil samples collected from gasoline and diesel stations. Bacteria were the most dominant microbiota and were therefore classified to generic level. Eleven main genera were detected and Corynebacterium was the predominant genus in all the samples. Biochemical characterisation and substrate utilisation showed high percentage of lipolytic ability combined with high inorganic nitrogen utilisers. The ability of these cultures to degrade crude oil was tested individually and in mixed bacterial consortium at different temperatures and pH values. Maximum crude oil biodegradation of 78% was achieved using a bacterial consortium containing five cultures (Micrococcus sp. GS2-22, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Bacillus sp. DS6-86 and Pseudomonas sp. DS10-129) with 1% crude oil at 30 degreesC and pH 7.5. Such a consortium may be useful for bioaugmentation of oil contaminated environments.

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