NUTRITIONAL-REQUIREMENTS AND GROWTH-CHARACTERISTICS OF A BIOSURFACTANT - PRODUCING RHODOCOCCUS BACTERIUM

AS Aburuwaida, Ibrahim Banat, S Haditirto, A Khamis

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Abstract

The nutritional requirements and growth characteristics of a biosurfactant-producing Rhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2% (v/v) n-paraffin as a carbon and energy source, 0.2% lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37-degrees-C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10(-3). Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.
LanguageEnglish
Pages53-61
JournalWorld Journal of Microbiology and Biotechnology
Volume7
Issue number1
Publication statusPublished - Jan 1991

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nutritional requirement
bacterium
surface tension
fermentation
surfactant
magnesium
metabolite
dilution
potassium
sodium
trace element
nitrate
phosphorus
iron
nitrogen
carbon
soil
temperature
biosynthesis
energy source

Cite this

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title = "NUTRITIONAL-REQUIREMENTS AND GROWTH-CHARACTERISTICS OF A BIOSURFACTANT - PRODUCING RHODOCOCCUS BACTERIUM",
abstract = "The nutritional requirements and growth characteristics of a biosurfactant-producing Rhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2{\%} (v/v) n-paraffin as a carbon and energy source, 0.2{\%} lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37-degrees-C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10(-3). Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.",
author = "AS Aburuwaida and Ibrahim Banat and S Haditirto and A Khamis",
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NUTRITIONAL-REQUIREMENTS AND GROWTH-CHARACTERISTICS OF A BIOSURFACTANT - PRODUCING RHODOCOCCUS BACTERIUM. / Aburuwaida, AS; Banat, Ibrahim; Haditirto, S; Khamis, A.

In: World Journal of Microbiology and Biotechnology, Vol. 7, No. 1, 01.1991, p. 53-61.

Research output: Contribution to journalArticle

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AB - The nutritional requirements and growth characteristics of a biosurfactant-producing Rhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2% (v/v) n-paraffin as a carbon and energy source, 0.2% lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37-degrees-C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10(-3). Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.

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