Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin

TB Chen, DF Orr, AJ Bjourson, Stephen McClean, M O'Rourke, DG Hirst, PF Rao, C Shaw

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Two novel bradykinin-related peptides (Ala3,Thr6)-bradykinin and (Val1,Thr3,Thr6)-bradykinin, were identified by a systematic sequencing study of peptides in the defensive skin secretion of the yellow-bellied toad, Bombina variegata . These peptides are the first amphibian skin bradykinins to exhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide. Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respectively, were cloned from a skin library by 3'- and 5'-RACE reactions. BVK-1 contained an open-reading frame of 97 amino acids encoding a single copy of (Ala3,Thr6)-bradykinin and similarly, the open-reading frame of BVK-2 consisted of 96 amino acids encoding a single copy of (Val1,Thr3,Thr6)-bradykinin. Synthetic replicates of each novel bradykinin were found to be active on mammalian arterial and small intestinal smooth muscle preparations. The structural diversity of bradykinins in amphibian defensive skin secretions may be related to defence against specific predators.
LanguageEnglish
Pages4693-4700
JournalEuropean Journal of Biochemistry
Volume269
Issue number18
DOIs
Publication statusPublished - Sep 2002

Fingerprint

Bradykinin
Anura
Complementary DNA
Skin
Amphibians
Peptides
Open Reading Frames
Amino Acids
Amino Acid Substitution
Smooth Muscle

Cite this

Chen, TB ; Orr, DF ; Bjourson, AJ ; McClean, Stephen ; O'Rourke, M ; Hirst, DG ; Rao, PF ; Shaw, C. / Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin. In: European Journal of Biochemistry. 2002 ; Vol. 269, No. 18. pp. 4693-4700.
@article{93e485e8e2a44a01ac1bb850e8c3fb4e,
title = "Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin",
abstract = "Two novel bradykinin-related peptides (Ala3,Thr6)-bradykinin and (Val1,Thr3,Thr6)-bradykinin, were identified by a systematic sequencing study of peptides in the defensive skin secretion of the yellow-bellied toad, Bombina variegata . These peptides are the first amphibian skin bradykinins to exhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide. Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respectively, were cloned from a skin library by 3'- and 5'-RACE reactions. BVK-1 contained an open-reading frame of 97 amino acids encoding a single copy of (Ala3,Thr6)-bradykinin and similarly, the open-reading frame of BVK-2 consisted of 96 amino acids encoding a single copy of (Val1,Thr3,Thr6)-bradykinin. Synthetic replicates of each novel bradykinin were found to be active on mammalian arterial and small intestinal smooth muscle preparations. The structural diversity of bradykinins in amphibian defensive skin secretions may be related to defence against specific predators.",
author = "TB Chen and DF Orr and AJ Bjourson and Stephen McClean and M O'Rourke and DG Hirst and PF Rao and C Shaw",
year = "2002",
month = "9",
doi = "10.1046/j.1432-1033.2002.03174.x",
language = "English",
volume = "269",
pages = "4693--4700",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
number = "18",

}

Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin. / Chen, TB; Orr, DF; Bjourson, AJ; McClean, Stephen; O'Rourke, M; Hirst, DG; Rao, PF; Shaw, C.

In: European Journal of Biochemistry, Vol. 269, No. 18, 09.2002, p. 4693-4700.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin

AU - Chen, TB

AU - Orr, DF

AU - Bjourson, AJ

AU - McClean, Stephen

AU - O'Rourke, M

AU - Hirst, DG

AU - Rao, PF

AU - Shaw, C

PY - 2002/9

Y1 - 2002/9

N2 - Two novel bradykinin-related peptides (Ala3,Thr6)-bradykinin and (Val1,Thr3,Thr6)-bradykinin, were identified by a systematic sequencing study of peptides in the defensive skin secretion of the yellow-bellied toad, Bombina variegata . These peptides are the first amphibian skin bradykinins to exhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide. Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respectively, were cloned from a skin library by 3'- and 5'-RACE reactions. BVK-1 contained an open-reading frame of 97 amino acids encoding a single copy of (Ala3,Thr6)-bradykinin and similarly, the open-reading frame of BVK-2 consisted of 96 amino acids encoding a single copy of (Val1,Thr3,Thr6)-bradykinin. Synthetic replicates of each novel bradykinin were found to be active on mammalian arterial and small intestinal smooth muscle preparations. The structural diversity of bradykinins in amphibian defensive skin secretions may be related to defence against specific predators.

AB - Two novel bradykinin-related peptides (Ala3,Thr6)-bradykinin and (Val1,Thr3,Thr6)-bradykinin, were identified by a systematic sequencing study of peptides in the defensive skin secretion of the yellow-bellied toad, Bombina variegata . These peptides are the first amphibian skin bradykinins to exhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide. Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respectively, were cloned from a skin library by 3'- and 5'-RACE reactions. BVK-1 contained an open-reading frame of 97 amino acids encoding a single copy of (Ala3,Thr6)-bradykinin and similarly, the open-reading frame of BVK-2 consisted of 96 amino acids encoding a single copy of (Val1,Thr3,Thr6)-bradykinin. Synthetic replicates of each novel bradykinin were found to be active on mammalian arterial and small intestinal smooth muscle preparations. The structural diversity of bradykinins in amphibian defensive skin secretions may be related to defence against specific predators.

U2 - 10.1046/j.1432-1033.2002.03174.x

DO - 10.1046/j.1432-1033.2002.03174.x

M3 - Article

VL - 269

SP - 4693

EP - 4700

JO - European Journal of Biochemistry

T2 - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 18

ER -