Nontargeted LC-MSn Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition.

GJ McDougall, JW Allwood, G Pereira-Caro, E Brown, Nigel G Ternan, S Verall, D Stewart, R Lawther, G O'Connor, I Rowland, A Crozier, Chris I R Gill

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ileostomy studies provide a unique insight into the digestion of foods, allowing identification of physiologically relevant dietary phytochemicals and their metabolites that are important to gut health. We previously reported an increase of components, including novel triterpenoids, in ileal fluids of 11 ileostomates following consumption of raspberries using nontargeted LC-MSn techniques in combination with data deconvolution software. The current study focused on components that consistently decreased postsupplementation. After data deconvolution, 32 components were identified that met exclusion parameters of m/z signals and which decreased significantly in ileal fluids from eight of 11 participants post-raspberry supplementation. Two-thirds of these components were identified putatively from their MS properties. Consistent decreases were observed in components that possibly reflected "washing out" of presupplementation intake of common foods/drinks including (poly)phenol metabolites. Metabolites associated with fat metabolism such as hydroxylated fatty acids and cholate-type bile acids were specifically reduced. However, more directed re-examination of the data revealed that although some cholates were consistently reduced, the more polar glyco- and tauro-linked bile acid derivatives increased consistently, by as much as 100-fold over presupplementation levels. The possible reasons for these substantial alterations in bile acid composition in ileal fluids in response to raspberry intake are discussed.
LanguageEnglish
Pages2606-2615
JournalJournal of Natural Products
Volume79
Issue number10
Early online date19 Sep 2016
DOIs
Publication statusE-pub ahead of print - 19 Sep 2016

Fingerprint

Bile Acids and Salts
Cholates
Ileostomy
Phytochemicals
Phenol
Digestion
Fatty Acids
Software
Eating
Fats
Food
Health
Rubus

Keywords

  • Raspberries
  • ileal fluid
  • bile acids

Cite this

McDougall, GJ ; Allwood, JW ; Pereira-Caro, G ; Brown, E ; Ternan, Nigel G ; Verall, S ; Stewart, D ; Lawther, R ; O'Connor, G ; Rowland, I ; Crozier, A ; Gill, Chris I R. / Nontargeted LC-MSn Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition. In: Journal of Natural Products. 2016 ; Vol. 79, No. 10. pp. 2606-2615.
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abstract = "Ileostomy studies provide a unique insight into the digestion of foods, allowing identification of physiologically relevant dietary phytochemicals and their metabolites that are important to gut health. We previously reported an increase of components, including novel triterpenoids, in ileal fluids of 11 ileostomates following consumption of raspberries using nontargeted LC-MSn techniques in combination with data deconvolution software. The current study focused on components that consistently decreased postsupplementation. After data deconvolution, 32 components were identified that met exclusion parameters of m/z signals and which decreased significantly in ileal fluids from eight of 11 participants post-raspberry supplementation. Two-thirds of these components were identified putatively from their MS properties. Consistent decreases were observed in components that possibly reflected {"}washing out{"} of presupplementation intake of common foods/drinks including (poly)phenol metabolites. Metabolites associated with fat metabolism such as hydroxylated fatty acids and cholate-type bile acids were specifically reduced. However, more directed re-examination of the data revealed that although some cholates were consistently reduced, the more polar glyco- and tauro-linked bile acid derivatives increased consistently, by as much as 100-fold over presupplementation levels. The possible reasons for these substantial alterations in bile acid composition in ileal fluids in response to raspberry intake are discussed.",
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McDougall, GJ, Allwood, JW, Pereira-Caro, G, Brown, E, Ternan, NG, Verall, S, Stewart, D, Lawther, R, O'Connor, G, Rowland, I, Crozier, A & Gill, CIR 2016, 'Nontargeted LC-MSn Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition.', Journal of Natural Products, vol. 79, no. 10, pp. 2606-2615. https://doi.org/10.1021/acs.jnatprod.6b00532

Nontargeted LC-MSn Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition. / McDougall, GJ; Allwood, JW; Pereira-Caro, G; Brown, E; Ternan, Nigel G; Verall, S; Stewart, D; Lawther, R; O'Connor, G; Rowland, I; Crozier, A; Gill, Chris I R.

In: Journal of Natural Products, Vol. 79, No. 10, 19.09.2016, p. 2606-2615.

Research output: Contribution to journalArticle

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T1 - Nontargeted LC-MSn Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition.

AU - McDougall, GJ

AU - Allwood, JW

AU - Pereira-Caro, G

AU - Brown, E

AU - Ternan, Nigel G

AU - Verall, S

AU - Stewart, D

AU - Lawther, R

AU - O'Connor, G

AU - Rowland, I

AU - Crozier, A

AU - Gill, Chris I R

N1 - Compliant in UIR; evidence uploaded to 'Other files'

PY - 2016/9/19

Y1 - 2016/9/19

N2 - Ileostomy studies provide a unique insight into the digestion of foods, allowing identification of physiologically relevant dietary phytochemicals and their metabolites that are important to gut health. We previously reported an increase of components, including novel triterpenoids, in ileal fluids of 11 ileostomates following consumption of raspberries using nontargeted LC-MSn techniques in combination with data deconvolution software. The current study focused on components that consistently decreased postsupplementation. After data deconvolution, 32 components were identified that met exclusion parameters of m/z signals and which decreased significantly in ileal fluids from eight of 11 participants post-raspberry supplementation. Two-thirds of these components were identified putatively from their MS properties. Consistent decreases were observed in components that possibly reflected "washing out" of presupplementation intake of common foods/drinks including (poly)phenol metabolites. Metabolites associated with fat metabolism such as hydroxylated fatty acids and cholate-type bile acids were specifically reduced. However, more directed re-examination of the data revealed that although some cholates were consistently reduced, the more polar glyco- and tauro-linked bile acid derivatives increased consistently, by as much as 100-fold over presupplementation levels. The possible reasons for these substantial alterations in bile acid composition in ileal fluids in response to raspberry intake are discussed.

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KW - Raspberries

KW - ileal fluid

KW - bile acids

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