Neuronal correlates of local, lateral, and translaminar inhibition with reference to cortical columns.

Moritz Helmstaedter, Bert Sakmann, Dirk Feldmeyer

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    In the neocortex, inhibition by gamma-aminobutyric acidergic (GABAergic) interneurons is essential for shaping cortical maps, which represent sensory signals. For a detailed understanding of the stream of excitation evoked, for example, by a sensory stimulus, interneurons must be identified with reference to their impact on excitatory neurons located in different laminae of the same (home) and surround columns. We analyzed the axonal projection of layer 2/3 (L2/3) interneurons with reference to geometric landmarks of cortical columns by staining neurons in acute slices of rat barrel cortex (P20-P29) and a subsequent cluster analysis using morphological parameters that described the spatial distribution of axons. The cluster analysis defined 4 main axonal projection "types" referred to as 1) "local inhibitors" (including "chandelier neurons"), 2) "lateral inhibitors," 3) "translaminar L2/3-to-L4/5 inhibitors," and 4) "translaminar L2/3-to-L1 inhibitors." The putative innervation domains established by axonal projections of the 4 types of interneurons and the dendritic domains of their target excitatory neurons were 1) L2/3 of the home column, 2) L2/3 of both the home and neighboring columns, 3) L4 and L5A of the home column, and 4) L1 and L2/3 of the home column. The quantitative analysis of the axonal projection patterns of an unselected sample of 51 interneurons located in L2/3 thus defined anatomical correlates for local, lateral, and translaminar inhibition within and between cortical columns.
    LanguageEnglish
    Pages926-937
    JournalCerebral Cortex
    Volume19
    Issue number4
    DOIs
    Publication statusPublished - Apr 2009

    Fingerprint

    Interneurons
    Neurons
    Cluster Analysis
    Neocortex
    Axons
    Staining and Labeling

    Keywords

    • Animals
    • Functional Laterality
    • physiology
    • Neural Inhibition
    • Neurons
    • cytology/physiology
    • Rats
    • Wistar
    • Somatosensory Cortex

    Cite this

    Helmstaedter, Moritz ; Sakmann, Bert ; Feldmeyer, Dirk. / Neuronal correlates of local, lateral, and translaminar inhibition with reference to cortical columns. In: Cerebral Cortex. 2009 ; Vol. 19, No. 4. pp. 926-937.
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    Neuronal correlates of local, lateral, and translaminar inhibition with reference to cortical columns. / Helmstaedter, Moritz; Sakmann, Bert; Feldmeyer, Dirk.

    In: Cerebral Cortex, Vol. 19, No. 4, 04.2009, p. 926-937.

    Research output: Contribution to journalArticle

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    T1 - Neuronal correlates of local, lateral, and translaminar inhibition with reference to cortical columns.

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    AU - Sakmann, Bert

    AU - Feldmeyer, Dirk

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    AB - In the neocortex, inhibition by gamma-aminobutyric acidergic (GABAergic) interneurons is essential for shaping cortical maps, which represent sensory signals. For a detailed understanding of the stream of excitation evoked, for example, by a sensory stimulus, interneurons must be identified with reference to their impact on excitatory neurons located in different laminae of the same (home) and surround columns. We analyzed the axonal projection of layer 2/3 (L2/3) interneurons with reference to geometric landmarks of cortical columns by staining neurons in acute slices of rat barrel cortex (P20-P29) and a subsequent cluster analysis using morphological parameters that described the spatial distribution of axons. The cluster analysis defined 4 main axonal projection "types" referred to as 1) "local inhibitors" (including "chandelier neurons"), 2) "lateral inhibitors," 3) "translaminar L2/3-to-L4/5 inhibitors," and 4) "translaminar L2/3-to-L1 inhibitors." The putative innervation domains established by axonal projections of the 4 types of interneurons and the dendritic domains of their target excitatory neurons were 1) L2/3 of the home column, 2) L2/3 of both the home and neighboring columns, 3) L4 and L5A of the home column, and 4) L1 and L2/3 of the home column. The quantitative analysis of the axonal projection patterns of an unselected sample of 51 interneurons located in L2/3 thus defined anatomical correlates for local, lateral, and translaminar inhibition within and between cortical columns.

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    KW - physiology

    KW - Neural Inhibition

    KW - Neurons

    KW - cytology/physiology

    KW - Rats

    KW - Wistar

    KW - Somatosensory Cortex

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