NATURAL-KILLER-CELL ACTIVITY IN LYMPHOCYTIC CHORIOMENINGITIS VIRUS-INFECTED BETA(2)-MICROGLOBULIN-DEFICIENT MICE

AJ ZAJAC, D MULLER, K PEDERSON, JA FRELINGER, Daniel Quinn

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    Abstract

    We have investigated the induction and role of natural killer (NK) activity in lymphocytic choriomeningitis virus (LCMV)-infected beta(2)-microgrobulin-deficient (beta(2)m(-)) mice. We demonstrate that LCMV infection is more effective than polyinosinic:polycytidylic acid (poly I:C) at stimulating NK activity in beta(2)m(-) mice. In addition, beta(2)m(-) NK cells respond poorly to in vitro treatment with IL-12. The target specificity of the virally induced NK cells is similar to that previously reported for chemically induced beta(2)m(-) NK cells. In both cases they can lyse YAC-1 tumor cells but are unable to kill beta(2)m(-) or beta(2)m(+) T cell blasts. We have also found that the time course of induction of MK and cytotoxic T lymphocyte (CTL) activity by LCMV in beta(2)m(-) mice is delayed compared with normal mice. Maximal NK and CTL activity is attained at day 8 and 10 post-infection respectively in beta(2)m(-) mice compared with day 4 and 6-8 in B6 mice. Whereas normal mice die similar to 7 days following intracranial infection with LCMV, the course of disease in beta(2)m(-) mice is protracted and characterized by a marked loss of body weight. We show that although the CD4(+) CTL response in these mice is intimately involved in mediating weight loss, the virus-induced NK cells do not appear to play a role in the disease.
    LanguageEnglish
    Pages1545-1556
    JournalInternational Immunology
    Volume7
    Issue number10
    DOIs
    Publication statusPublished - Oct 1995

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    Lymphocytic choriomeningitis virus
    beta 2-Microglobulin
    Natural Killer Cells
    Cytotoxic T-Lymphocytes
    Virus Diseases
    Poly I-C
    Interleukin-12
    Infection
    Weight Loss
    Body Weight
    Viruses
    T-Lymphocytes

    Cite this

    ZAJAC, AJ ; MULLER, D ; PEDERSON, K ; FRELINGER, JA ; Quinn, Daniel. / NATURAL-KILLER-CELL ACTIVITY IN LYMPHOCYTIC CHORIOMENINGITIS VIRUS-INFECTED BETA(2)-MICROGLOBULIN-DEFICIENT MICE. In: International Immunology. 1995 ; Vol. 7, No. 10. pp. 1545-1556.
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    abstract = "We have investigated the induction and role of natural killer (NK) activity in lymphocytic choriomeningitis virus (LCMV)-infected beta(2)-microgrobulin-deficient (beta(2)m(-)) mice. We demonstrate that LCMV infection is more effective than polyinosinic:polycytidylic acid (poly I:C) at stimulating NK activity in beta(2)m(-) mice. In addition, beta(2)m(-) NK cells respond poorly to in vitro treatment with IL-12. The target specificity of the virally induced NK cells is similar to that previously reported for chemically induced beta(2)m(-) NK cells. In both cases they can lyse YAC-1 tumor cells but are unable to kill beta(2)m(-) or beta(2)m(+) T cell blasts. We have also found that the time course of induction of MK and cytotoxic T lymphocyte (CTL) activity by LCMV in beta(2)m(-) mice is delayed compared with normal mice. Maximal NK and CTL activity is attained at day 8 and 10 post-infection respectively in beta(2)m(-) mice compared with day 4 and 6-8 in B6 mice. Whereas normal mice die similar to 7 days following intracranial infection with LCMV, the course of disease in beta(2)m(-) mice is protracted and characterized by a marked loss of body weight. We show that although the CD4(+) CTL response in these mice is intimately involved in mediating weight loss, the virus-induced NK cells do not appear to play a role in the disease.",
    author = "AJ ZAJAC and D MULLER and K PEDERSON and JA FRELINGER and Daniel Quinn",
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    language = "English",
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    NATURAL-KILLER-CELL ACTIVITY IN LYMPHOCYTIC CHORIOMENINGITIS VIRUS-INFECTED BETA(2)-MICROGLOBULIN-DEFICIENT MICE. / ZAJAC, AJ; MULLER, D; PEDERSON, K; FRELINGER, JA; Quinn, Daniel.

    In: International Immunology, Vol. 7, No. 10, 10.1995, p. 1545-1556.

    Research output: Contribution to journalArticle

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