Nanoplasmonics for Real-Time and Label-Free Monitoring of Microbial Biofilm Formation

Riccardo Funari, Nikhil Bhalla, Kang Yu Chu, Bill Söderström, Amy Q. Shen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Microbial biofilms possess intrinsic resistance against conventional antibiotics and cleaning procedures; thus, a better understanding of their complex biological structures is crucial in both medical and industrial applications. Existing laboratory methodologies have focused on macroscopic and mostly indirect characterization of mechanical and microbiological properties of biofilms adhered on a given substrate. However, the kinetics underlying the biofilm formation is not well understood, while such information is critical to understanding how drugs and chemicals influence the biofilm formation. Herein, we report the use of localized surface plasmon resonance (LSPR) for real-time, label-free monitoring of E. coli biofilm assembly on a nanoplasmonic substrate consisting of gold mushroom-like structures. Our LSPR sensor is able to capture the signatures of biofilm formation in real-time by measuring the wavelength shift in the LSPR resonance peak with high temporal resolution. We employ this sensor feature to elucidate how biofilm formation is affected by different drugs, including conventional antibiotics (kanamycin and ampicillin) as well as rifapentine, a molecule preventing cell adhesion yet barely affecting bacterial viability and vitality. Due to its flexibility and simplicity, our LSPR based platform can be used on a wide variety of clinically relevant bacteria, thus representing a valuable tool in biofilm characterization and drug screening.

LanguageEnglish
Pages1499-1509
Number of pages11
JournalACS Sensors
Volume3
Issue number8
Early online date31 Jul 2018
DOIs
Publication statusPublished - 24 Aug 2018

Fingerprint

biofilms
Biofilms
Labels
Surface Plasmon Resonance
Monitoring
Surface plasmon resonance
surface plasmon resonance
rifapentine
drugs
antibiotics
Antibiotics
Pharmaceutical Preparations
Anti-Bacterial Agents
Microbial Viability
Preclinical Drug Evaluations
Kanamycin
Agaricales
sensors
Cell adhesion
Sensors

Keywords

  • antibiotics
  • biofilms
  • drug screening
  • E. coli
  • LSPR
  • real-time monitoring

Cite this

Funari, Riccardo ; Bhalla, Nikhil ; Chu, Kang Yu ; Söderström, Bill ; Shen, Amy Q. / Nanoplasmonics for Real-Time and Label-Free Monitoring of Microbial Biofilm Formation. In: ACS Sensors. 2018 ; Vol. 3, No. 8. pp. 1499-1509.
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Nanoplasmonics for Real-Time and Label-Free Monitoring of Microbial Biofilm Formation. / Funari, Riccardo; Bhalla, Nikhil; Chu, Kang Yu; Söderström, Bill; Shen, Amy Q.

In: ACS Sensors, Vol. 3, No. 8, 24.08.2018, p. 1499-1509.

Research output: Contribution to journalArticle

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