Muscarinic Acetylcholine Receptor-Mediated Phosphoinositide Turnover in Cultured Human Retinal Pigment Epithelium Cells.

NN Osborne, F FitzGibbon, G Schwartz

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Cultured retinal pigment epithelium cells prepared from post-mortem adult human eyes are shown to contain muscarinic receptors associated with phosphoinositide turnover. Carbachol at a concentration of 100μM induced a four-fold increase in3H-inositol phosphates (more than 76% is in the form of3H-inositol-1-phosphate) accumulation within 45 min in cells prelabelled with3H-myoinositol and exposed to 5mM LiCl. The EC50 of carbachol was approx. 70μM and the saturation concentration was about 1 mM. The carbachol-induced response was blocked by both atropine and pirenzepine, the former being most effective. Pre-exposure of cells to carbachol resulted in desensitization and a drastic reduction in the subsequent carbachol-induced stimulation of3H-inositol phosphates. The carbachol response could be attenuated by the biologically active phorbol ester, 4β-phorbol 12-myristate 13-acetate, and this was nullified by the protein kinase C inhibitor, staurosporine. The biologically inactive phorbol ester, 4α-phorbol 12,13 dideconoate, did not attenuate the carbachol-induced stimulation of3H-inositol phosphates. Pertussis toxin failed to influence the carbachol receptor-mediated phosphoinositide turnover. These studies provide clear evidence for the occurrence of muscarinic receptors coupled to phosphoinositide hydrolysis on human retinal pigment epithelium cells.
    Original languageEnglish
    Pages (from-to)1119-1127
    JournalVision Research
    Volume31
    Issue number7-8
    DOIs
    Publication statusPublished - 1991

    Keywords

    • Phosphoinositide turnover
    • pigment epithelium cells
    • cholinergic receptors

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