Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays

A.A. Al-Fadda, A.A. Turjoman, K. Newell, D.S. Gibson, J.V. Lamont, M.W. Duncan

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Background. Variations in the levels of adiponectin, C-peptide, CRP, cystatin C, ferritin, IL-1α, IL-6, insulin, leptin, PAI-1, resistin,TNFα have been associated to metabolic dysfunctions. Biochip array technology enables their multiplex determination from asingle sample, at a single point in time. This generates a patient’s profile, which provides more information than single analytedetermination. The aim of this study was to determine these analytes in plasma of obese subjects following weight loss.Methods. Plasma samples from obese subjects (n=12, average IBM>30kg/m2) were analysed. For weight reduction, 5 subjectsfollowed dietary restriction and 7 subjects underwent bariatric surgery. Two different biochip arrays were used for the multiplex determination.Simultaneous chemiluminescent immunoassays applied to the Evidence Investigator analyser were used. Statisticalsignificance between groups was calculated using the paired Student’s t-test (two tailed).Results. Following dietary restriction, significant reductions were observed in plasma insulin and CRP levels(p=0.045 and 0.030respectively). C-peptide, IL-6, leptin and TNFα exhibited moderate but no statistically significant reduction. A marginal increasewas observed in PAI-1 and resistin. Adiponectin and IL-1α remained unchanged. Following bariatric surgery, a highly significantreduction was observed in plasma leptin(p=0.005) and a significant increase was observed in plasma adiponectin(p=0.014). Cpeptideand PAI-1 were reduced and IL-1α, resistin and TNFα increased but did not reach statistical significance. No significantchanges were detected in CRP, IL-6 and insulin levels.Conclusions. This multi-analytical approach using biochip arrays shows differences between the groups undergoing weight lossand represents a valuable analytical tool in research settings.
    LanguageEnglish
    Title of host publicationUnknown Host Publication
    PagesO385
    Number of pages1
    Volume49
    Publication statusPublished - 2011
    EventIFCC – WorldLab – EuroMedLab - Berlin
    Duration: 1 Jan 2011 → …

    Conference

    ConferenceIFCC – WorldLab – EuroMedLab
    Period1/01/11 → …

    Fingerprint

    Weight Loss
    Resistin
    Adiponectin
    Plasminogen Activator Inhibitor 1
    Leptin
    Interleukin-6
    Bariatric Surgery
    C-Peptide
    Insulin
    Cystatin C
    Ferritins
    Immunoassay
    Research Personnel
    Students
    Technology
    Weights and Measures
    Research

    Keywords

    • Metabolic Syndrome
    • Weight loss
    • protein array
    • cytokines

    Cite this

    Al-Fadda, A. A., Turjoman, A. A., Newell, K., Gibson, D. S., Lamont, J. V., & Duncan, M. W. (2011). Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays. In Unknown Host Publication (Vol. 49, pp. O385)
    Al-Fadda, A.A. ; Turjoman, A.A. ; Newell, K. ; Gibson, D.S. ; Lamont, J.V. ; Duncan, M.W. / Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays. Unknown Host Publication. Vol. 49 2011. pp. O385
    @inproceedings{7be7f1495fb44593914a0d8da5a59f81,
    title = "Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays",
    abstract = "Background. Variations in the levels of adiponectin, C-peptide, CRP, cystatin C, ferritin, IL-1α, IL-6, insulin, leptin, PAI-1, resistin,TNFα have been associated to metabolic dysfunctions. Biochip array technology enables their multiplex determination from asingle sample, at a single point in time. This generates a patient’s profile, which provides more information than single analytedetermination. The aim of this study was to determine these analytes in plasma of obese subjects following weight loss.Methods. Plasma samples from obese subjects (n=12, average IBM>30kg/m2) were analysed. For weight reduction, 5 subjectsfollowed dietary restriction and 7 subjects underwent bariatric surgery. Two different biochip arrays were used for the multiplex determination.Simultaneous chemiluminescent immunoassays applied to the Evidence Investigator analyser were used. Statisticalsignificance between groups was calculated using the paired Student’s t-test (two tailed).Results. Following dietary restriction, significant reductions were observed in plasma insulin and CRP levels(p=0.045 and 0.030respectively). C-peptide, IL-6, leptin and TNFα exhibited moderate but no statistically significant reduction. A marginal increasewas observed in PAI-1 and resistin. Adiponectin and IL-1α remained unchanged. Following bariatric surgery, a highly significantreduction was observed in plasma leptin(p=0.005) and a significant increase was observed in plasma adiponectin(p=0.014). Cpeptideand PAI-1 were reduced and IL-1α, resistin and TNFα increased but did not reach statistical significance. No significantchanges were detected in CRP, IL-6 and insulin levels.Conclusions. This multi-analytical approach using biochip arrays shows differences between the groups undergoing weight lossand represents a valuable analytical tool in research settings.",
    keywords = "Metabolic Syndrome, Weight loss, protein array, cytokines",
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    year = "2011",
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    Al-Fadda, AA, Turjoman, AA, Newell, K, Gibson, DS, Lamont, JV & Duncan, MW 2011, Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays. in Unknown Host Publication. vol. 49, pp. O385, IFCC – WorldLab – EuroMedLab, 1/01/11.

    Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays. / Al-Fadda, A.A.; Turjoman, A.A.; Newell, K.; Gibson, D.S.; Lamont, J.V.; Duncan, M.W.

    Unknown Host Publication. Vol. 49 2011. p. O385.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    TY - GEN

    T1 - Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays

    AU - Al-Fadda, A.A.

    AU - Turjoman, A.A.

    AU - Newell, K.

    AU - Gibson, D.S.

    AU - Lamont, J.V.

    AU - Duncan, M.W.

    PY - 2011

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    N2 - Background. Variations in the levels of adiponectin, C-peptide, CRP, cystatin C, ferritin, IL-1α, IL-6, insulin, leptin, PAI-1, resistin,TNFα have been associated to metabolic dysfunctions. Biochip array technology enables their multiplex determination from asingle sample, at a single point in time. This generates a patient’s profile, which provides more information than single analytedetermination. The aim of this study was to determine these analytes in plasma of obese subjects following weight loss.Methods. Plasma samples from obese subjects (n=12, average IBM>30kg/m2) were analysed. For weight reduction, 5 subjectsfollowed dietary restriction and 7 subjects underwent bariatric surgery. Two different biochip arrays were used for the multiplex determination.Simultaneous chemiluminescent immunoassays applied to the Evidence Investigator analyser were used. Statisticalsignificance between groups was calculated using the paired Student’s t-test (two tailed).Results. Following dietary restriction, significant reductions were observed in plasma insulin and CRP levels(p=0.045 and 0.030respectively). C-peptide, IL-6, leptin and TNFα exhibited moderate but no statistically significant reduction. A marginal increasewas observed in PAI-1 and resistin. Adiponectin and IL-1α remained unchanged. Following bariatric surgery, a highly significantreduction was observed in plasma leptin(p=0.005) and a significant increase was observed in plasma adiponectin(p=0.014). Cpeptideand PAI-1 were reduced and IL-1α, resistin and TNFα increased but did not reach statistical significance. No significantchanges were detected in CRP, IL-6 and insulin levels.Conclusions. This multi-analytical approach using biochip arrays shows differences between the groups undergoing weight lossand represents a valuable analytical tool in research settings.

    AB - Background. Variations in the levels of adiponectin, C-peptide, CRP, cystatin C, ferritin, IL-1α, IL-6, insulin, leptin, PAI-1, resistin,TNFα have been associated to metabolic dysfunctions. Biochip array technology enables their multiplex determination from asingle sample, at a single point in time. This generates a patient’s profile, which provides more information than single analytedetermination. The aim of this study was to determine these analytes in plasma of obese subjects following weight loss.Methods. Plasma samples from obese subjects (n=12, average IBM>30kg/m2) were analysed. For weight reduction, 5 subjectsfollowed dietary restriction and 7 subjects underwent bariatric surgery. Two different biochip arrays were used for the multiplex determination.Simultaneous chemiluminescent immunoassays applied to the Evidence Investigator analyser were used. Statisticalsignificance between groups was calculated using the paired Student’s t-test (two tailed).Results. Following dietary restriction, significant reductions were observed in plasma insulin and CRP levels(p=0.045 and 0.030respectively). C-peptide, IL-6, leptin and TNFα exhibited moderate but no statistically significant reduction. A marginal increasewas observed in PAI-1 and resistin. Adiponectin and IL-1α remained unchanged. Following bariatric surgery, a highly significantreduction was observed in plasma leptin(p=0.005) and a significant increase was observed in plasma adiponectin(p=0.014). Cpeptideand PAI-1 were reduced and IL-1α, resistin and TNFα increased but did not reach statistical significance. No significantchanges were detected in CRP, IL-6 and insulin levels.Conclusions. This multi-analytical approach using biochip arrays shows differences between the groups undergoing weight lossand represents a valuable analytical tool in research settings.

    KW - Metabolic Syndrome

    KW - Weight loss

    KW - protein array

    KW - cytokines

    M3 - Conference contribution

    VL - 49

    SP - O385

    BT - Unknown Host Publication

    ER -

    Al-Fadda AA, Turjoman AA, Newell K, Gibson DS, Lamont JV, Duncan MW. Multiplex determination of analytes related to metabolic syndrome after weight loss with Evidence biochip arrays. In Unknown Host Publication. Vol. 49. 2011. p. O385