Multimodal electrochemical and nanoplasmonic biosensors using ferrocene-crowned nanoparticles for kinase drug discovery applications

Nello Formisano, Nikhil Bhalla, L. C.Caleb Wong, Mirella Di Lorenzo, Giordano Pula, Pedro Estrela

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.

LanguageEnglish
Pages70-73
Number of pages4
JournalElectrochemistry Communications
Volume57
Issue number1
DOIs
Publication statusPublished - 29 May 2015

Fingerprint

Biosensors
Phosphotransferases
Nanoparticles
Surface plasmon resonance
Biomolecules
Voltammetry
Gold
Drug products
Screening
Spectroscopy
Proteins
Networks (circuits)
Pharmaceutical Preparations
ferrocene
Drug Discovery

Keywords

  • Amperometric sensing
  • Electrochemical impedance
  • Localised surface plasmon resonance
  • Multimodal biosensor
  • Open circuit potential
  • Protein phosphorylation

Cite this

Formisano, Nello ; Bhalla, Nikhil ; Wong, L. C.Caleb ; Di Lorenzo, Mirella ; Pula, Giordano ; Estrela, Pedro. / Multimodal electrochemical and nanoplasmonic biosensors using ferrocene-crowned nanoparticles for kinase drug discovery applications. In: Electrochemistry Communications. 2015 ; Vol. 57, No. 1. pp. 70-73.
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Multimodal electrochemical and nanoplasmonic biosensors using ferrocene-crowned nanoparticles for kinase drug discovery applications. / Formisano, Nello; Bhalla, Nikhil; Wong, L. C.Caleb; Di Lorenzo, Mirella; Pula, Giordano; Estrela, Pedro.

In: Electrochemistry Communications, Vol. 57, No. 1, 29.05.2015, p. 70-73.

Research output: Contribution to journalArticle

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