Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles

Nello Formisano, Nikhil Bhalla, L. C.Caleb Wong, Pedro Estrela

Research output: Contribution to conferenceAbstract

Abstract

The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.
Original languageEnglish
DOIs
Publication statusPublished - 29 May 2015
Event4th International Conference on Bio-Sensing Technologies - , Portugal
Duration: 10 May 201513 May 2015

Conference

Conference4th International Conference on Bio-Sensing Technologies
CountryPortugal
Period10/05/1513/05/15

Fingerprint Dive into the research topics of 'Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles'. Together they form a unique fingerprint.

Cite this