Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles

Nello Formisano, Nikhil Bhalla, L. C.Caleb Wong, Pedro Estrela

Research output: Contribution to conferenceAbstract

Abstract

The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.

Conference

Conference4th International Conference on Bio-Sensing Technologies
CountryPortugal
Period10/05/1513/05/15

Fingerprint

Phosphotransferases
Nanoparticles
Surface plasmon resonance
Biomolecules
Voltammetry
Biosensors
Gold
Screening
Spectroscopy
Networks (circuits)
Pharmaceutical Preparations
Proteins
ferrocene
Drug Discovery

Cite this

Formisano, N., Bhalla, N., Wong, L. C. C., & Estrela, P. (2015). Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles. Abstract from 4th International Conference on Bio-Sensing Technologies, Portugal. https://doi.org/10.1016/j.elecom.2015.05.014
Formisano, Nello ; Bhalla, Nikhil ; Wong, L. C.Caleb ; Estrela, Pedro. / Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles. Abstract from 4th International Conference on Bio-Sensing Technologies, Portugal.
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title = "Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles",
abstract = "The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.",
author = "Nello Formisano and Nikhil Bhalla and Wong, {L. C.Caleb} and Pedro Estrela",
year = "2015",
month = "5",
day = "29",
doi = "10.1016/j.elecom.2015.05.014",
language = "English",
note = "4th International Conference on Bio-Sensing Technologies ; Conference date: 10-05-2015 Through 13-05-2015",

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Formisano, N, Bhalla, N, Wong, LCC & Estrela, P 2015, 'Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles' 4th International Conference on Bio-Sensing Technologies, Portugal, 10/05/15 - 13/05/15, . https://doi.org/10.1016/j.elecom.2015.05.014

Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles. / Formisano, Nello; Bhalla, Nikhil; Wong, L. C.Caleb; Estrela, Pedro.

2015. Abstract from 4th International Conference on Bio-Sensing Technologies, Portugal.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles

AU - Formisano, Nello

AU - Bhalla, Nikhil

AU - Wong, L. C.Caleb

AU - Estrela, Pedro

PY - 2015/5/29

Y1 - 2015/5/29

N2 - The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.

AB - The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report, we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely, differential pulse voltammetry, impedance spectroscopy, and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localised surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental, and pharmaceutical applications.

U2 - 10.1016/j.elecom.2015.05.014

DO - 10.1016/j.elecom.2015.05.014

M3 - Abstract

ER -

Formisano N, Bhalla N, Wong LCC, Estrela P. Multi-mode electrochemical biosensing for kinase drug discovery applications using ferrocene crowned nanoparticles. 2015. Abstract from 4th International Conference on Bio-Sensing Technologies, Portugal. https://doi.org/10.1016/j.elecom.2015.05.014