Multi-method biophysical analysis in discovery, identification, and in-depth characterization of surface‐active compounds

Aikaterini Zompra, Styliani A Chasapi, Matthew Twigg, Karina Salek, Ioannis Anestopoulos, Alex Galanis, Aglaia Pappa, Tony Gutierrez, Ibrahim M Banat, R Marchant, Stephen R Euston, Mihalis Panayiotidis, Georgios Spyroulias

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4 Citations (Scopus)
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Synthetic surfactants are used in several industries, including manufacturing, pharmaceutical and cosmetic’s, food and feed, agriculture, petroleum and environmental remediation for their ability to adsorb to fluid and solid-water interfaces. However, their widespread use and their synthetic preparation through environmentally unfavorable processes counterbalances the value of this class of reagents. This fact has stimulated new efforts to exploit natural sources of surfactants, such as new classes of bacterial systems or manipulation of existing biological systems, that may produce, through an environmentally friendly process, new biodegradable surfactants and emulsifiers of high commercial value. A downside of microbial production of biobased chemicals such as these types of chemicals, is that their fermentation often yields crude materials consisting of several bioproducts with complex physical and chemical properties. Extraction, identification, and efficient characterization of biosurfactants from a crude mixture of biomolecules requires carefully designed, and detailed analytical processes using state-of-the-art methods. The purpose of this review article is to present the current state-of-the-art and future outlook on the various multidisciplinary biophysical methods applied in the discovery, extraction identification, and in-depth characterization of microbially-produced surface‐active compounds.
Original languageEnglish
Article number1023287
Pages (from-to)1-18
Number of pages18
JournalFrontiers in Marine Science
Volume 9
Early online date20 Oct 2022
Publication statusPublished (in print/issue) - 20 Oct 2022

Bibliographical note

Funding Information:
This research was funded by the European Union Framework Programme for Research and Innovation, Horizon 2020, under Grant Agreement No. 635340 MARISURF. This was also supported by the INSPIRED (MIS 5002550) which is implemented under the Action ‘Reinforcement of the Research and Innovation Infrastructure,’ funded by the Operational Program ‘Competitiveness, Entrepreneurship and Innovation’ (NSRF 2014–2020) and co-financed by Greece and the European Union (European Regional Development Fund). EU FP7 REGPOT CT-2011-285950 – “SEE-DRUG” project is acknowledged for the purchase of UPAT’s 700 MHz NMR equipment.

Publisher Copyright:
Copyright © 2022 Zompra, Chasapi, Twigg, Salek, Anestopoulos, Galanis, Pappa, Gutierrez, Banat, Marchant, Euston, Panayiotidis and Spyroulias.


  • biosurfactants
  • biophysical analysis
  • NMR
  • LC-MS
  • HPLC
  • SEC


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