Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

L. Clarke; J. E. Moore; B. C. Millar; M. Crowe; J. Xu; C. E. Goldsmith; P. G. Murphy; James Dooley; J. Rendall; J. S. Elborn

Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

L. Clarke, J. E. Moore, B. C. Millar, M. Crowe, J. Xu, C. E. Goldsmith, P. G. Murphy, James Dooley, J. Rendall, J. S. Elborn

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11 Citations (Scopus)

Abstract

Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.

Original language	English
Pages (from-to)	18-21
Journal	British Journal of Biomedical Science
Volume	65
Issue number	1
Publication status	Published (in print/issue) - 2008

Cite this

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title = "Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland",

abstract = "Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.",

author = "L. Clarke and Moore, {J. E.} and Millar, {B. C.} and M. Crowe and J. Xu and Goldsmith, {C. E.} and Murphy, {P. G.} and James Dooley and J. Rendall and Elborn, {J. S.}",

year = "2008",

language = "English",

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TY - JOUR

T1 - Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

AU - Clarke, L.

AU - Moore, J. E.

AU - Millar, B. C.

AU - Crowe, M.

AU - Xu, J.

AU - Goldsmith, C. E.

AU - Murphy, P. G.

AU - Dooley, James

AU - Rendall, J.

AU - Elborn, J. S.

PY - 2008

Y1 - 2008

N2 - Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.

AB - Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.

M3 - Article

SN - 2474-0896

VL - 65

SP - 18

EP - 21

JO - British Journal of Biomedical Science

JF - British Journal of Biomedical Science

IS - 1

ER -

Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

Abstract

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