Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

L. Clarke, J. E. Moore, B. C. Millar, M. Crowe, J. Xu, C. E. Goldsmith, P. G. Murphy, James Dooley, J. Rendall, J. S. Elborn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.
LanguageEnglish
Pages18-21
JournalBritish Journal of Biomedical Science
Volume65
Issue number1
Publication statusPublished - 2008

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Northern Ireland
Molecular Epidemiology
Cystic Fibrosis
Pseudomonas aeruginosa
Genotyping Techniques
Intergenic DNA
Genetic Heterogeneity
Consensus Sequence
DNA-Directed DNA Polymerase
Sputum
Polymerase Chain Reaction

Cite this

Clarke, L., Moore, J. E., Millar, B. C., Crowe, M., Xu, J., Goldsmith, C. E., ... Elborn, J. S. (2008). Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland. British Journal of Biomedical Science, 65(1), 18-21.
Clarke, L. ; Moore, J. E. ; Millar, B. C. ; Crowe, M. ; Xu, J. ; Goldsmith, C. E. ; Murphy, P. G. ; Dooley, James ; Rendall, J. ; Elborn, J. S. / Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland. In: British Journal of Biomedical Science. 2008 ; Vol. 65, No. 1. pp. 18-21.
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abstract = "Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.",
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Clarke, L, Moore, JE, Millar, BC, Crowe, M, Xu, J, Goldsmith, CE, Murphy, PG, Dooley, J, Rendall, J & Elborn, JS 2008, 'Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland', British Journal of Biomedical Science, vol. 65, no. 1, pp. 18-21.

Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland. / Clarke, L.; Moore, J. E.; Millar, B. C.; Crowe, M.; Xu, J.; Goldsmith, C. E.; Murphy, P. G.; Dooley, James; Rendall, J.; Elborn, J. S.

In: British Journal of Biomedical Science, Vol. 65, No. 1, 2008, p. 18-21.

Research output: Contribution to journalArticle

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T1 - Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

AU - Clarke, L.

AU - Moore, J. E.

AU - Millar, B. C.

AU - Crowe, M.

AU - Xu, J.

AU - Goldsmith, C. E.

AU - Murphy, P. G.

AU - Dooley, James

AU - Rendall, J.

AU - Elborn, J. S.

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AB - Isolates (n = 51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.

M3 - Article

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SP - 18

EP - 21

JO - British Journal of Biomedical Science

T2 - British Journal of Biomedical Science

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