Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland

A. Tazumi, Y. Maeda, T. Buckley, B. C. Millar, C. E. Goldsmith, James Dooley, J. S. Elborn, M. Matsuda, J. E. Moore

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Clinical isolates (n=63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n=24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPID PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPID PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.
LanguageEnglish
Pages456-459
JournalIrish Veterinary Journal
Volume62
Issue number7
Publication statusPublished - Jul 2009

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Molecular Epidemiology
Ireland
Pseudomonas aeruginosa
Horses
Genotype
Disease Outbreaks
Genotyping Techniques
Random Amplified Polymorphic DNA Technique
Molecular Typing
Polymerase Chain Reaction
Genetic Heterogeneity

Cite this

Tazumi, A. ; Maeda, Y. ; Buckley, T. ; Millar, B. C. ; Goldsmith, C. E. ; Dooley, James ; Elborn, J. S. ; Matsuda, M. ; Moore, J. E. / Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland. In: Irish Veterinary Journal. 2009 ; Vol. 62, No. 7. pp. 456-459.
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abstract = "Clinical isolates (n=63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n=24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9{\%}) of the isolates. ERIC2 RAPID PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPID PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.",
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Tazumi, A, Maeda, Y, Buckley, T, Millar, BC, Goldsmith, CE, Dooley, J, Elborn, JS, Matsuda, M & Moore, JE 2009, 'Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland', Irish Veterinary Journal, vol. 62, no. 7, pp. 456-459.

Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland. / Tazumi, A.; Maeda, Y.; Buckley, T.; Millar, B. C.; Goldsmith, C. E.; Dooley, James; Elborn, J. S.; Matsuda, M.; Moore, J. E.

In: Irish Veterinary Journal, Vol. 62, No. 7, 07.2009, p. 456-459.

Research output: Contribution to journalArticle

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AB - Clinical isolates (n=63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n=24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPID PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPID PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.

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