TY - JOUR
T1 - Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus:a randomised controlled trial
AU - Davison, Gareth
AU - Ashton, Tony
AU - George, Lindsay
AU - Young, Ian
AU - Davies, Bruce
AU - Jackson, Simon
AU - Peters, John
AU - Bailey, Damian
PY - 2008
Y1 - 2008
N2 - Aims/hypothesis Patients with type 1 diabetes mellitus aremore susceptible than healthy individuals to exercise-inducedoxidative stress and vascular endothelial dysfunction, whichhas important implications for the progression of disease.Thus, in the present study, we designed a randomised doubleblind,placebo-controlled trial to test the original hypothesisthat oral prophylaxis with vitamin C attenuates rest andexercise-induced free radical-mediated lipid peroxidation intype 1 diabetes mellitus.Methods All data were collected from hospitalised diabeticpatients. The electron paramagnetic resonance spectroscopicdetection of spin-trapped α-phenyl-tert-butylnitrone(PBN) adducts was combined with the use of supportingmarkers of lipid peroxidation and non-enzymatic antioxidantsto assess exercise-induced oxidative stress in malepatients with type 1 diabetes (HbA1c 7.9±1%, n=12) andhealthy controls (HbA1c 4.6±0.5%, n=14). Followingparticipant randomisation using numbers in a sealed envelope,venous blood samples were obtained at rest, after a maximalexercise challenge and before and 2 h after oral ingestion of1 g ascorbate or placebo. Participants and lead investigatorswere blinded to the administration of either placebo orascorbate treatments. Primary outcome was the difference inchanges in free radicals following ascorbate ingestion.Results Six diabetic patients and seven healthy controlparticipants were randomised to each of the placebo andascorbate groups. Diabetic patients (n=12) exhibited anelevated concentration of PBN adducts (p
AB - Aims/hypothesis Patients with type 1 diabetes mellitus aremore susceptible than healthy individuals to exercise-inducedoxidative stress and vascular endothelial dysfunction, whichhas important implications for the progression of disease.Thus, in the present study, we designed a randomised doubleblind,placebo-controlled trial to test the original hypothesisthat oral prophylaxis with vitamin C attenuates rest andexercise-induced free radical-mediated lipid peroxidation intype 1 diabetes mellitus.Methods All data were collected from hospitalised diabeticpatients. The electron paramagnetic resonance spectroscopicdetection of spin-trapped α-phenyl-tert-butylnitrone(PBN) adducts was combined with the use of supportingmarkers of lipid peroxidation and non-enzymatic antioxidantsto assess exercise-induced oxidative stress in malepatients with type 1 diabetes (HbA1c 7.9±1%, n=12) andhealthy controls (HbA1c 4.6±0.5%, n=14). Followingparticipant randomisation using numbers in a sealed envelope,venous blood samples were obtained at rest, after a maximalexercise challenge and before and 2 h after oral ingestion of1 g ascorbate or placebo. Participants and lead investigatorswere blinded to the administration of either placebo orascorbate treatments. Primary outcome was the difference inchanges in free radicals following ascorbate ingestion.Results Six diabetic patients and seven healthy controlparticipants were randomised to each of the placebo andascorbate groups. Diabetic patients (n=12) exhibited anelevated concentration of PBN adducts (p
U2 - 10.1007/s00125-008-1101-1
DO - 10.1007/s00125-008-1101-1
M3 - Article
SN - 1432-0428
VL - 51
SP - 2049
EP - 2059
JO - Diabetologia
JF - Diabetologia
ER -
