Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus:a randomised controlled trial

Gareth Davison, Tony Ashton, Lindsay George, Ian Young, Bruce Davies, Simon Jackson, John Peters, Damian Bailey

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Aims/hypothesis Patients with type 1 diabetes mellitus aremore susceptible than healthy individuals to exercise-inducedoxidative stress and vascular endothelial dysfunction, whichhas important implications for the progression of disease.Thus, in the present study, we designed a randomised doubleblind,placebo-controlled trial to test the original hypothesisthat oral prophylaxis with vitamin C attenuates rest andexercise-induced free radical-mediated lipid peroxidation intype 1 diabetes mellitus.Methods All data were collected from hospitalised diabeticpatients. The electron paramagnetic resonance spectroscopicdetection of spin-trapped α-phenyl-tert-butylnitrone(PBN) adducts was combined with the use of supportingmarkers of lipid peroxidation and non-enzymatic antioxidantsto assess exercise-induced oxidative stress in malepatients with type 1 diabetes (HbA1c 7.9±1%, n=12) andhealthy controls (HbA1c 4.6±0.5%, n=14). Followingparticipant randomisation using numbers in a sealed envelope,venous blood samples were obtained at rest, after a maximalexercise challenge and before and 2 h after oral ingestion of1 g ascorbate or placebo. Participants and lead investigatorswere blinded to the administration of either placebo orascorbate treatments. Primary outcome was the difference inchanges in free radicals following ascorbate ingestion.Results Six diabetic patients and seven healthy controlparticipants were randomised to each of the placebo andascorbate groups. Diabetic patients (n=12) exhibited anelevated concentration of PBN adducts (p
Original languageEnglish
Pages (from-to)2049-2059
JournalDiabetologia
Volume51
DOIs
Publication statusPublished - 2008

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