Abstract
Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SglI in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ~4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.
| Original language | English |
|---|---|
| Pages (from-to) | 295-299 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 394 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published (in print/issue) - 7 Oct 1996 |
Funding
Acknowledgements: This work was supported by grants from IN-SERM (U413) and the Conseil R6gional de Haute-Normandie. We thank Dr. T.B. Usdin (Lab. Cell Biology, NIMH, NIH) for the preparation of the frog pituitary library and P. Bizet for excellent technical assistance. Y.A. was a recipient of a postdoctoral fellowship from the Conseil R~gional de Haute-Normandie.
| Funder number |
|---|
| U413 |
Keywords
- Chromogranin
- Frog
- Peptide precursor
- Secretogranin II
- Secretoneurin
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