Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides

Youssef Anouar; Sylvie Jégou; David Alexandre; Isabelle Lihrmann; J. Michael Conlon; Hubert Vaudry

doi:10.1016/0014-5793(96)00976-3

Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides

Youssef Anouar, Sylvie Jégou, David Alexandre, Isabelle Lihrmann, J. Michael Conlon, Hubert Vaudry

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SglI in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ~4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	FEBS Letters
Volume	394
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(96)00976-3
Publication status	Published (in print/issue) - 7 Oct 1996

Keywords

Chromogranin
Frog
Peptide precursor
Secretogranin II
Secretoneurin

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10.1016/0014-5793(96)00976-3

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title = "Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides",

abstract = "Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SglI in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ~4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.",

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author = "Youssef Anouar and Sylvie J{\'e}gou and David Alexandre and Isabelle Lihrmann and Conlon, {J. Michael} and Hubert Vaudry",

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AU - Anouar, Youssef

AU - Jégou, Sylvie

AU - Alexandre, David

AU - Lihrmann, Isabelle

AU - Conlon, J. Michael

AU - Vaudry, Hubert

PY - 1996/10/7

Y1 - 1996/10/7

N2 - Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SglI in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ~4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.

AB - Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SglI in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46-48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68-82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ~4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.

KW - Chromogranin

KW - Frog

KW - Peptide precursor

KW - Secretogranin II

KW - Secretoneurin

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AN - SCOPUS:0030573996

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JF - FEBS Letters

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ER -

Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides

Abstract

Keywords

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