MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE

L BUNNI, TJ HACKETT, L MCHALE, G FLYNN, Anthony McHale

Research output: Contribution to journalArticle

Abstract

A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.
LanguageEnglish
Pages1095-1100
JournalBiotechnology Letters
Volume15
Issue number11
DOIs
Publication statusPublished - Nov 1993

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Talaromyces
alpha-Amylases
Molecular Cloning
Complementary DNA
HeLa Cells
Cell Line
Amylases
Northern Blotting
Glucocorticoids
Fungi
Messenger RNA
Acids
DNA
Enzymes

Cite this

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title = "MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE",
abstract = "A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.",
author = "L BUNNI and TJ HACKETT and L MCHALE and G FLYNN and Anthony McHale",
year = "1993",
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MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE. / BUNNI, L; HACKETT, TJ; MCHALE, L; FLYNN, G; McHale, Anthony.

In: Biotechnology Letters, Vol. 15, No. 11, 11.1993, p. 1095-1100.

Research output: Contribution to journalArticle

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AU - McHale, Anthony

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