MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE

L BUNNI; TJ HACKETT; L MCHALE; G FLYNN; Anthony McHale

doi:10.1007/BF00131196

MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE

L BUNNI, TJ HACKETT, L MCHALE, G FLYNN, Anthony McHale

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.

Original language	English
Pages (from-to)	1095-1100
Journal	Biotechnology Letters
Volume	15
Issue number	11
DOIs	https://doi.org/10.1007/BF00131196
Publication status	Published (in print/issue) - Nov 1993

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title = "MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE",

abstract = "A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.",

author = "L BUNNI and TJ HACKETT and L MCHALE and G FLYNN and Anthony McHale",

year = "1993",

month = nov,

doi = "10.1007/BF00131196",

language = "English",

volume = "15",

pages = "1095--1100",

journal = "Biotechnology Letters",

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publisher = "Springer",

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AU - BUNNI, L

AU - HACKETT, TJ

AU - MCHALE, L

AU - FLYNN, G

AU - McHale, Anthony

PY - 1993/11

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N2 - A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.

AB - A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.

U2 - 10.1007/BF00131196

DO - 10.1007/BF00131196

M3 - Article

SN - 1573-6776

VL - 15

SP - 1095

EP - 1100

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 11

ER -

MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE

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