Molecular characterisation of Alternaria linicola and its detection in linseed

GJ McKay, AE Brown, AJ Bjourson, PC Mercer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nucleotide sequences of the ribosomal DNA(rDNA) internal transcribed spacers (ITS) 1 and 2 and a 1068 bp section of the beta-tubulin gene divided seven designated species of Alternaria into five taxa. Stemphylium botryosum formed a sixth closely related taxon. Isolates of A. linicola possessed an identical ITS sequence to one group of A. solani isolates, and two clusters of A. linicola isolates, revealed from beta-tubulin gene data to show minor variation, were as genetically similar to isolates of A. solani as they were to each other. We suggest, therefore, that A. linicola falls within the species A. solani. Similar results suggest that A. lini falls within the species A. alternata. RAPD analysis of the total genomic DNA from the Alternaria spp. concurred with the nucleotide sequence analyses. An oligonucleotide primer (ALP) was selected from the rDNA ITS1 region of A. linicola/A. solani. PCR with primers ALP and ITS4 (from a conserved region of the rDNA) amplified a c. 536 bp fragment from isolates of A. linicola and A. solani but not from other Alternaria spp. nor from other fungi which may be associated with linseed. These primers amplified an identical fragment, confirmed by Southern hybridization, from DNA released from infected linseed seed and leaf tissues. These primers have the potential to be used also for the detection of A. solani in host tissues.
LanguageEnglish
Pages157-166
JournalEuropean Journal of Plant Pathology
Volume105
Issue number2
Publication statusPublished - Feb 1999

Fingerprint

Alternaria
linseed
ribosomal DNA
DNA primers
tubulin
internal transcribed spacers
Stemphylium
Alternaria solani
nucleotide sequences
DNA
Southern blotting
genes
genomics
fungi
seeds
leaves
tissues

Cite this

@article{f26d06a9b5bd4e0ea42a02725bee07c2,
title = "Molecular characterisation of Alternaria linicola and its detection in linseed",
abstract = "Nucleotide sequences of the ribosomal DNA(rDNA) internal transcribed spacers (ITS) 1 and 2 and a 1068 bp section of the beta-tubulin gene divided seven designated species of Alternaria into five taxa. Stemphylium botryosum formed a sixth closely related taxon. Isolates of A. linicola possessed an identical ITS sequence to one group of A. solani isolates, and two clusters of A. linicola isolates, revealed from beta-tubulin gene data to show minor variation, were as genetically similar to isolates of A. solani as they were to each other. We suggest, therefore, that A. linicola falls within the species A. solani. Similar results suggest that A. lini falls within the species A. alternata. RAPD analysis of the total genomic DNA from the Alternaria spp. concurred with the nucleotide sequence analyses. An oligonucleotide primer (ALP) was selected from the rDNA ITS1 region of A. linicola/A. solani. PCR with primers ALP and ITS4 (from a conserved region of the rDNA) amplified a c. 536 bp fragment from isolates of A. linicola and A. solani but not from other Alternaria spp. nor from other fungi which may be associated with linseed. These primers amplified an identical fragment, confirmed by Southern hybridization, from DNA released from infected linseed seed and leaf tissues. These primers have the potential to be used also for the detection of A. solani in host tissues.",
author = "GJ McKay and AE Brown and AJ Bjourson and PC Mercer",
year = "1999",
month = "2",
language = "English",
volume = "105",
pages = "157--166",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
number = "2",

}

Molecular characterisation of Alternaria linicola and its detection in linseed. / McKay, GJ; Brown, AE; Bjourson, AJ; Mercer, PC.

In: European Journal of Plant Pathology, Vol. 105, No. 2, 02.1999, p. 157-166.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular characterisation of Alternaria linicola and its detection in linseed

AU - McKay, GJ

AU - Brown, AE

AU - Bjourson, AJ

AU - Mercer, PC

PY - 1999/2

Y1 - 1999/2

N2 - Nucleotide sequences of the ribosomal DNA(rDNA) internal transcribed spacers (ITS) 1 and 2 and a 1068 bp section of the beta-tubulin gene divided seven designated species of Alternaria into five taxa. Stemphylium botryosum formed a sixth closely related taxon. Isolates of A. linicola possessed an identical ITS sequence to one group of A. solani isolates, and two clusters of A. linicola isolates, revealed from beta-tubulin gene data to show minor variation, were as genetically similar to isolates of A. solani as they were to each other. We suggest, therefore, that A. linicola falls within the species A. solani. Similar results suggest that A. lini falls within the species A. alternata. RAPD analysis of the total genomic DNA from the Alternaria spp. concurred with the nucleotide sequence analyses. An oligonucleotide primer (ALP) was selected from the rDNA ITS1 region of A. linicola/A. solani. PCR with primers ALP and ITS4 (from a conserved region of the rDNA) amplified a c. 536 bp fragment from isolates of A. linicola and A. solani but not from other Alternaria spp. nor from other fungi which may be associated with linseed. These primers amplified an identical fragment, confirmed by Southern hybridization, from DNA released from infected linseed seed and leaf tissues. These primers have the potential to be used also for the detection of A. solani in host tissues.

AB - Nucleotide sequences of the ribosomal DNA(rDNA) internal transcribed spacers (ITS) 1 and 2 and a 1068 bp section of the beta-tubulin gene divided seven designated species of Alternaria into five taxa. Stemphylium botryosum formed a sixth closely related taxon. Isolates of A. linicola possessed an identical ITS sequence to one group of A. solani isolates, and two clusters of A. linicola isolates, revealed from beta-tubulin gene data to show minor variation, were as genetically similar to isolates of A. solani as they were to each other. We suggest, therefore, that A. linicola falls within the species A. solani. Similar results suggest that A. lini falls within the species A. alternata. RAPD analysis of the total genomic DNA from the Alternaria spp. concurred with the nucleotide sequence analyses. An oligonucleotide primer (ALP) was selected from the rDNA ITS1 region of A. linicola/A. solani. PCR with primers ALP and ITS4 (from a conserved region of the rDNA) amplified a c. 536 bp fragment from isolates of A. linicola and A. solani but not from other Alternaria spp. nor from other fungi which may be associated with linseed. These primers amplified an identical fragment, confirmed by Southern hybridization, from DNA released from infected linseed seed and leaf tissues. These primers have the potential to be used also for the detection of A. solani in host tissues.

M3 - Article

VL - 105

SP - 157

EP - 166

JO - European Journal of Plant Pathology

T2 - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 2

ER -