MiR-182 Is Upregulated in Prostate Cancer and Contributes to Tumor Progression by Targeting MITF

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)
39 Downloads (Pure)

Abstract

Altered expression of microRNA-182-5p (miR-182) has been consistently linked with many cancers, but its specific role in prostate cancer remains unclear. In particular, its contribution to epithelial–to–mesenchymal transition (EMT) in this setting has not been well studied. Therefore, this paper profiles the expression of miR-182 in prostate cancer and investigates how it may contribute to progression of this disease. In vitro experiments on prostate cancer cell lines and in silico analyses of The Cancer Genome Atlas (TCGA) prostate adenocarcinoma (PRAD) datasets were performed. PCR revealed miR-182 expression was significantly increased in prostate cancer cell lines compared to normal prostate cells. Bioinformatic analysis of TCGA PRAD data similarly showed upregulation of miR-182 was significantly associated with prostate cancer and clinical markers of disease progression. Functional enrichment analysis confirmed a significant association of miR-182 and its target genes with EMT. The EMT-linked gene MITF (melanocyte inducing transcription factor) was subsequently shown to be a novel target of miR-182 in prostate cancer cells. Further TCGA analysis suggested miR-182 expression can be an indicator of patient outcomes and disease progression following therapy. In summary, this is the first study to report that miR-182 over-expression in prostate cancer may contribute to EMT by targeting MITF expression. We propose miR-182 as a potentially useful diagnostic and prognostic biomarker for prostate cancer and other malignancies.
Original languageEnglish
Article number1824
Number of pages16
JournalInternational Journal of Molecular Sciences
Volume24
Issue number3
DOIs
Publication statusPublished (in print/issue) - 17 Jan 2023

Bibliographical note

Funding Information:
This research was funded by the Department for the Economy (DfE2019), Northern Ireland, as a PhD studentship secured by D.J.M. and awarded to M.Y.C.S.

Publisher Copyright:
© 2023 by the authors.

Keywords

  • Article
  • prostate cancer
  • microRNA
  • miR-182
  • epithelial-to-mesenchymal transition
  • MITF
  • biomarker
  • Epithelial-to-mesenchymal Transition
  • Up-Regulation
  • Biomarker
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Male
  • Mitf
  • Disease Progression
  • Microphthalmia-Associated Transcription Factor
  • Prostatic Neoplasms
  • MicroRNA
  • MicroRNAs
  • Epithelial-Mesenchymal Transition
  • Cell Line, Tumor
  • Mir-182
  • Cell Movement

Fingerprint

Dive into the research topics of 'MiR-182 Is Upregulated in Prostate Cancer and Contributes to Tumor Progression by Targeting MITF'. Together they form a unique fingerprint.

Cite this