TY - GEN
T1 - Microparticle filtration using carbon nanotubes and impedance characterisation for gold microelectrodes sensor system
AU - Mathur, A.
AU - Hamad, E. M.
AU - Mukhopadhyay, S.
AU - Roy, S. S.
AU - McLaughlin, J. A.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Blood-cell-free serum is required for most clinical chemistry tests. At present bend micro channel and polymeric pillars are used in polymer based microfluidic devices (such as PMMA) for the blood filtration. In this study, we have fabricated carbon nanotube (CNT) pillars on silicon from 20-50 μm in diameter with ∼10 μm spacing and integrate them inside the microfluidic channel with a view of using these for blood plasma filtration from whole blood, with passive capillary flow. Our main objective is to design a novel sensor, comprising CNT arrays, to filter/control whole blood flow, with an integrated micro patterned gold electrode which will be sealed by bonding into microfluidics structures. We have characterized the microfluidic channel by measuring the meniscus movement profiles. Also gold inter-digitated electrodes (IDEs) were fabricated on glass and immobilized with an antibody. These IDEs were used as an impedance-based biosensor using label-free antigen - antibody interaction. At a fixed frequency, the IDEs gave a linear response across the range of concentrations of secondary antibodies investigated (0 to 500 μg/mL).
AB - Blood-cell-free serum is required for most clinical chemistry tests. At present bend micro channel and polymeric pillars are used in polymer based microfluidic devices (such as PMMA) for the blood filtration. In this study, we have fabricated carbon nanotube (CNT) pillars on silicon from 20-50 μm in diameter with ∼10 μm spacing and integrate them inside the microfluidic channel with a view of using these for blood plasma filtration from whole blood, with passive capillary flow. Our main objective is to design a novel sensor, comprising CNT arrays, to filter/control whole blood flow, with an integrated micro patterned gold electrode which will be sealed by bonding into microfluidics structures. We have characterized the microfluidic channel by measuring the meniscus movement profiles. Also gold inter-digitated electrodes (IDEs) were fabricated on glass and immobilized with an antibody. These IDEs were used as an impedance-based biosensor using label-free antigen - antibody interaction. At a fixed frequency, the IDEs gave a linear response across the range of concentrations of secondary antibodies investigated (0 to 500 μg/mL).
KW - Bio-sensing
KW - Carbon nanotubes (CNTs)
KW - Hot embossing
KW - Poly methyl meth acralate (PMMA)
UR - http://www.scopus.com/inward/record.url?scp=79952065373&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:79952065373
SN - 9781617822025
T3 - Materials Research Society Symposium Proceedings
SP - 32
EP - 37
BT - Large-Area Electronics from Carbon Nanotubes, Graphene, and Related Noncarbon Nanostructures
T2 - 2009 MRS Fall Meeting
Y2 - 30 November 2009 through 4 December 2009
ER -