Microfluidic platform for enzyme-linked and magnetic particle-based immunoassay

Nikhil Bhalla, Danny Wen Yaw Chung, Yaw Jen Chang, Kimberly Jane S Uy, Yi Ying Ye, Ting Yu Chin, Hao Chun Yang, Dorota G. Pijanowska

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead-protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.

Original languageEnglish
Pages (from-to)257-271
Number of pages15
Issue number2
Early online date18 Jun 2013
Publication statusPublished (in print/issue) - 14 Oct 2013


  • Bead-protein complex
  • Bio-MEMS
  • Hall Effect
  • Immunoassay
  • Magnetic bead
  • Microfluidics


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