Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug

Elisabeth Kastner, Varun Verma, Deborah Lowry, Yvonne Perrie

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.
    LanguageEnglish
    Pages122-130
    JournalInternational Journal of Pharmaceutics
    Volume485
    Issue number1-2
    Early online date25 May 2015
    DOIs
    Publication statusE-pub ahead of print - 25 May 2015

    Fingerprint

    Microfluidics
    Liposomes
    Water
    Pharmaceutical Preparations
    Propofol
    Solubility
    Technology
    Costs and Cost Analysis

    Keywords

    • Liposomes
    • Microfluidics
    • Poorly soluble drugs
    • Bilayer loading
    • High throughput

    Cite this

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    abstract = "Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol{\%}) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.",
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    Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. / Kastner, Elisabeth; Verma, Varun; Lowry, Deborah; Perrie, Yvonne.

    In: International Journal of Pharmaceutics, Vol. 485, No. 1-2, 25.05.2015, p. 122-130.

    Research output: Contribution to journalArticle

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    AU - Verma, Varun

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    AB - Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

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