TY - JOUR
T1 - Microbially mediated phenolic catabolites exert differential genoprotective activities in normal and adenocarcinoma cell lines
AU - Murphy, Brian Óg
AU - Latimer, Cheryl
AU - Dobani, Sara
AU - Pourshahidi, L. Kirsty
AU - Fontana, Massimilano
AU - Ternan, Nigel. G.
AU - McDougall, Gordon
AU - Rowland, Ian
AU - Pereira-Caro, Gema
AU - Tuohy, Kieran M.
AU - Del Rio, Daniele
AU - Almutairi, Tahani M.
AU - Crozier, Alan
AU - Naumovski, Nenad
AU - Gill, Chris I. R.
N1 - Publisher Copyright:
© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2024/9/11
Y1 - 2024/9/11
N2 - Age-associated decline of nuclear factor erythroid 2-related factor 2 (Nrf2) activity and DNA repair efficiency leads to the accumulation of DNA damage and increased risk of cancer. Understanding the mechanisms behind increased levels of damaged DNA is crucial for developing interventions to mitigate age-related cancer risk. Associated with various health benefits, (poly)phenols and their microbially mediated phenolic catabolites represent a potential means to reduce DNA damage. Four colonic-microbiota-derived phenolic catabolites were investigated for their ability to reduce H2O2-induced oxidative DNA damage and modulate the Nrf2-Antixoidant Response Element (ARE) pathway, in normal (CCD 841 CoN) and adenocarcinoma (HT29) colonocyte cell lines. Each catabolite demonstrated significant (p < .001) genoprotective activity and modulation of key genes in the Nrf2-ARE pathway. Overall, the colon-derived phenolic metabolites, when assessed at physiologically relevant concentrations, reduced DNA damage in both normal and adenocarcinoma colonic cells in response to oxidative challenge, mediated in part via upregulation of the Nrf2-ARE pathway.
AB - Age-associated decline of nuclear factor erythroid 2-related factor 2 (Nrf2) activity and DNA repair efficiency leads to the accumulation of DNA damage and increased risk of cancer. Understanding the mechanisms behind increased levels of damaged DNA is crucial for developing interventions to mitigate age-related cancer risk. Associated with various health benefits, (poly)phenols and their microbially mediated phenolic catabolites represent a potential means to reduce DNA damage. Four colonic-microbiota-derived phenolic catabolites were investigated for their ability to reduce H2O2-induced oxidative DNA damage and modulate the Nrf2-Antixoidant Response Element (ARE) pathway, in normal (CCD 841 CoN) and adenocarcinoma (HT29) colonocyte cell lines. Each catabolite demonstrated significant (p < .001) genoprotective activity and modulation of key genes in the Nrf2-ARE pathway. Overall, the colon-derived phenolic metabolites, when assessed at physiologically relevant concentrations, reduced DNA damage in both normal and adenocarcinoma colonic cells in response to oxidative challenge, mediated in part via upregulation of the Nrf2-ARE pathway.
KW - Benzoic acid
KW - colonic cells
KW - COMET assay
KW - 4-hydroxybenzoic acid
KW - 3-(3′-hydroxyphenyl)propanoic acid
KW - 3-(phenyl)propanoic acid
UR - https://pure.ulster.ac.uk/en/publications/6ea08c05-e0cb-46e6-8caa-a04c23f59e1c
UR - http://www.scopus.com/inward/record.url?scp=85203554914&partnerID=8YFLogxK
U2 - 10.1080/09637486.2024.2397055
DO - 10.1080/09637486.2024.2397055
M3 - Article
C2 - 39261459
SN - 0963-7486
SP - 1
EP - 14
JO - International Journal of Food Sciences and Nutrition
JF - International Journal of Food Sciences and Nutrition
ER -
