Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples

Research output: Contribution to conferencePoster

1 Citation (Scopus)

Abstract

It is well established that drinking water contaminated with faecal matter poses a series risk of waterborne disease yet, it is estimated that almost 2 million people are reliant on such sources. The production of improved water sources for low- and middle-income countries has been a long-standing international commitment but ensuring that the drinking water is safe to drink remains a challenge – particularly in rural communities. The development of low-cost diagnostic systems capable of detecting coliform contamination is clearly required. The design of a disposable microfluidic based device that integrates the preconcentration, culture and detection of E. coli is presented as a possible solution. The device is based on a thin layer conductive carbon fibre filter modified with a riboflavin – ferrocyanide redox couple. The device utilises the fermentation characteristics of captured indicator bacteria to lower the local pH by the production of lactic acid and pyruvate after the introduction of lactose. The process can be monitored voltammetrically whereby ferrocyanide (internal reference) and riboflavin (pH probe) are readily detectable at the carbon fibre filter and allows the indirect measure of the change in pH as a function of time and bacterial concentration. The construction of the device and the electroanalytical responses are presented and the analytical efficacy, in terms of bacterial detection and in relation to present WHO criteria, is critically evaluated.

Conference

ConferenceMicrobiology Society Annual Conference 2019
CountryUnited Kingdom
CityBelfast
Period8/04/1911/04/19
Internet address

Fingerprint

Riboflavin
Potable water
Drinking Water
Carbon fibers
Water quality
Lactic acid
Lactose
Fermentation
Escherichia coli
Lactic Acid
Bacteria
Water
Costs
hexacyanoferrate II
carbon fiber
Oxidation-Reduction

Keywords

  • Coliform
  • Galactosidase
  • Riboflavin
  • Sensor
  • Water quality
  • pH

Cite this

@conference{a06ec8e3c3554a618bb2602c79da7d54,
title = "Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples",
abstract = "It is well established that drinking water contaminated with faecal matter poses a series risk of waterborne disease yet, it is estimated that almost 2 million people are reliant on such sources. The production of improved water sources for low- and middle-income countries has been a long-standing international commitment but ensuring that the drinking water is safe to drink remains a challenge – particularly in rural communities. The development of low-cost diagnostic systems capable of detecting coliform contamination is clearly required. The design of a disposable microfluidic based device that integrates the preconcentration, culture and detection of E. coli is presented as a possible solution. The device is based on a thin layer conductive carbon fibre filter modified with a riboflavin – ferrocyanide redox couple. The device utilises the fermentation characteristics of captured indicator bacteria to lower the local pH by the production of lactic acid and pyruvate after the introduction of lactose. The process can be monitored voltammetrically whereby ferrocyanide (internal reference) and riboflavin (pH probe) are readily detectable at the carbon fibre filter and allows the indirect measure of the change in pH as a function of time and bacterial concentration. The construction of the device and the electroanalytical responses are presented and the analytical efficacy, in terms of bacterial detection and in relation to present WHO criteria, is critically evaluated.",
keywords = "Coliform, Galactosidase, Riboflavin, Sensor, Water quality, pH",
author = "Teri Bigham and Charnete Casimero and J Dooley and Ternan, {Nigel G} and W Snelling and J Davis",
year = "2019",
language = "English",
pages = "99--103",
note = "Microbiology Society Annual Conference 2019 ; Conference date: 08-04-2019 Through 11-04-2019",
url = "https://microbiologysociety.org/event/annual-conference/annual-conference.html",

}

Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples. / Bigham, Teri; Casimero, Charnete; Dooley, J; Ternan, Nigel G; Snelling, W; Davis, J.

2019. 99-103 Poster session presented at Microbiology Society Annual Conference 2019, Belfast, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples

AU - Bigham, Teri

AU - Casimero, Charnete

AU - Dooley, J

AU - Ternan, Nigel G

AU - Snelling, W

AU - Davis, J

PY - 2019

Y1 - 2019

N2 - It is well established that drinking water contaminated with faecal matter poses a series risk of waterborne disease yet, it is estimated that almost 2 million people are reliant on such sources. The production of improved water sources for low- and middle-income countries has been a long-standing international commitment but ensuring that the drinking water is safe to drink remains a challenge – particularly in rural communities. The development of low-cost diagnostic systems capable of detecting coliform contamination is clearly required. The design of a disposable microfluidic based device that integrates the preconcentration, culture and detection of E. coli is presented as a possible solution. The device is based on a thin layer conductive carbon fibre filter modified with a riboflavin – ferrocyanide redox couple. The device utilises the fermentation characteristics of captured indicator bacteria to lower the local pH by the production of lactic acid and pyruvate after the introduction of lactose. The process can be monitored voltammetrically whereby ferrocyanide (internal reference) and riboflavin (pH probe) are readily detectable at the carbon fibre filter and allows the indirect measure of the change in pH as a function of time and bacterial concentration. The construction of the device and the electroanalytical responses are presented and the analytical efficacy, in terms of bacterial detection and in relation to present WHO criteria, is critically evaluated.

AB - It is well established that drinking water contaminated with faecal matter poses a series risk of waterborne disease yet, it is estimated that almost 2 million people are reliant on such sources. The production of improved water sources for low- and middle-income countries has been a long-standing international commitment but ensuring that the drinking water is safe to drink remains a challenge – particularly in rural communities. The development of low-cost diagnostic systems capable of detecting coliform contamination is clearly required. The design of a disposable microfluidic based device that integrates the preconcentration, culture and detection of E. coli is presented as a possible solution. The device is based on a thin layer conductive carbon fibre filter modified with a riboflavin – ferrocyanide redox couple. The device utilises the fermentation characteristics of captured indicator bacteria to lower the local pH by the production of lactic acid and pyruvate after the introduction of lactose. The process can be monitored voltammetrically whereby ferrocyanide (internal reference) and riboflavin (pH probe) are readily detectable at the carbon fibre filter and allows the indirect measure of the change in pH as a function of time and bacterial concentration. The construction of the device and the electroanalytical responses are presented and the analytical efficacy, in terms of bacterial detection and in relation to present WHO criteria, is critically evaluated.

KW - Coliform

KW - Galactosidase

KW - Riboflavin

KW - Sensor

KW - Water quality

KW - pH

M3 - Poster

SP - 99

EP - 103

ER -