Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples

Research output: Contribution to journalArticle

Abstract

The ability to screen water for the presence of faecal contamination is a pressing need for rural communities dependent upon local purification systems. While there are a multitude of coliform detection assays based on the activity of β-galactosidase, this report details the adaptation of a voltammetric pH sensing strategy which could offer rapid analysis. The approach exploits the bacterial metabolism of lactose via pyruvate to lactate with the subsequent decrease in pH measured by examining the peak separation of a riboflavin (sensing)-ferrocyanide (reference) couple. Disposable carbon fibre electrodes were used as in situ sensors in Escherichia coli cultures (103–107 cfu/mL) with detection times of 4h enabling confirmation of coliform activity. The bacterial compatibility
of the riboflavin–ferrocyanide system in combination with the simplicity of the detection methodology, stand in marked contrast to many existing coliform assays and could open new avenues through which voltammetric
pH sensing could be employed.
LanguageEnglish
Pages99-103
Number of pages5
JournalElectrochemistry Communications
Volume101
Early online date1 Mar 2019
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

Riboflavin
Water quality
Assays
Lactose
beta-Galactosidase
Pyruvic Acid
Metabolism
Escherichia coli
Carbon fibers
Purification
Lactic Acid
Contamination
Electrodes
Water
Sensors
hexacyanoferrate II
Oxidation-Reduction
carbon fiber
Galactosidases

Keywords

  • Galactosidase, pH, Riboflavin, Coliform, Water quality, Sensor
  • Water quality
  • Coliform
  • Galactosidase
  • pH
  • Riboflavin
  • Sensor

Cite this

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title = "Microbial water quality: Voltammetric detection of coliforms based on riboflavin–ferrocyanide redox couples",
abstract = "The ability to screen water for the presence of faecal contamination is a pressing need for rural communities dependent upon local purification systems. While there are a multitude of coliform detection assays based on the activity of β-galactosidase, this report details the adaptation of a voltammetric pH sensing strategy which could offer rapid analysis. The approach exploits the bacterial metabolism of lactose via pyruvate to lactate with the subsequent decrease in pH measured by examining the peak separation of a riboflavin (sensing)-ferrocyanide (reference) couple. Disposable carbon fibre electrodes were used as in situ sensors in Escherichia coli cultures (103–107 cfu/mL) with detection times of 4h enabling confirmation of coliform activity. The bacterial compatibilityof the riboflavin–ferrocyanide system in combination with the simplicity of the detection methodology, stand in marked contrast to many existing coliform assays and could open new avenues through which voltammetricpH sensing could be employed.",
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author = "Teri Bigham and Charnete Casimero and James Dooley and Ternan, {Nigel G} and Snelling, {William J} and J Davis",
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AU - Ternan, Nigel G

AU - Snelling, William J

AU - Davis, J

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